【摘要】 ①目的观察孕酮对大鼠脑损伤后环氧化酶-2表达和脑水肿的影响。②方法雄性SD大鼠随机分为假手术组、脑损伤组、安慰剂组和孕酮治疗组。按照改良的Feeney自由落体损伤装置制作大鼠脑损伤模型。各组于伤后1、24、72小时取材。用免疫组织化学法观察大鼠皮质COX-2的表达水平变化,并测量脑组织含水量。③结果脑损伤组和安慰剂组大鼠皮质COX-2阳性细胞数和脑水肿较假手术组显著增加;孕酮治疗组与脑损伤组和安慰剂组比较,大鼠皮质COX-2阳性细胞数和脑水肿明显减少,差异有统计学意义(P<0.05)。④结论孕酮可能通过下调COX-2的表达,降低脑内炎症反应,进而降低脑水肿,发挥脑损伤保护作用。更多还原

【Abstract】 Objective To investigate effects of progesterone treatment on COX-2 expression and brain edema after traumatic brain injury in rats. Methods Male Spraque- Dawley rats were randomly divided sham- operated group,TBI group,Vehicle and PROG- treated group. The rat model of TBI was duplicated with the improved Feeney’s method. The rats were killed respectively at 1,24,72 after injury. The changes of the positive cell numbers of COX- 2 were examined by immunohistochemistry and brain edema were measured. Results The positive cell numbers of COX- 2 and brain edema of the TBI and Vehicle group were increased. The positive cell numbers of COX- 2 and brain edema of the PROG- treated group were distinctly less than those of the TBI and Vehicle group( P < 0. 05). Conclusion The protective effect of progesterone on brain edema may be induced by down-regulation of COX- 2 expression and reduce inflammation after traumatic brain injury.更多还原