【摘要】 目的探讨COX-2抑制剂对膀胱癌T24细胞株裸鼠成瘤性的影响。方法 BALB/c裸鼠27只分为3组,每只皮下接种膀胱癌T24细胞5×106活细胞数建立移植瘤动物模型。COX-2抑制剂药物干预组(吲哚美辛、塞来昔布)采用喂饲途径,吲哚美辛3mg/kg,塞来昔布10mg/kg;对照组给予生理盐水溶液药物的投给。30d后处死裸鼠,取瘤块称重,测量肿瘤体积,行免疫组化、半定量RT-PCR、Wester Blot检测移植瘤COX-2表达。结果对照组细胞接种裸鼠后第5天可见肿瘤长出,第7天各组均有肿瘤长出,第30天后用药组移植瘤生长较对照组明显减慢。免疫组化染色、RT-PCR、Western-blot结果均显示对照组COX-2表达明显,而药物干预组均少量表达。结论选择性与非选择性COX-2抑制剂在实验中表现出良好的抗肿瘤特性。更多还原

【Abstract】 Objective To establish the nude mice xenograft models of bladder cancer cell T24 and to study the inhibitive effects of Cyclooxygenase-2(COX-2).Methods Each of 27 athymic BALB/c mice was injected with 5×106 T24 bladder cancer cells.The mice were then divided equally into three groups.One group received oral indomethacin 3 mg/kg per mouse daily,the other 10 mg/kg celecoxib per mouse daily,and the control group received normal saline.The nude mice were killed after 30 days,and the weight and volume of xenografts were recorded.Histological hematoxylin-osin staining and immunocytochemical SABC technique were employed to explore the histopathological changes.The expression of COX-2 in the xenografts was detected by RT-PCR and Western-blot.Results On the 5th day after transplantation,xenografts were found in the control group.On the 7th day,xenografts were observed in the other two groups.The expression level of COX-2 reduced and the weight and volume of the xenografts decreased more in the groups treated with indomethacin or celecoxib. Conclusions Selective and non-selective COX-2 inhibitors can inhibit the growth of xenografts in nude mice.更多还原