【摘要】 采用高效液相色谱法同时测定楠竹叶提取物中的4种主要黄酮成分:荭草苷、牡荆苷、牡荆苷-4′-O-葡萄糖苷、牡荆苷-2′-O-鼠李糖苷。通过实验,得到了同时测定4种主要黄酮成分的最佳条件。采用Kromasil-C18（5μm,4.6 mm×250 mm,Akzol Nobel,Sweden）进行测定,最佳检测条件为:以四氢呋喃:乙腈:甲醇:0.5%醋酸水溶液=10.3:1:0.7:88为色谱流动相等度洗脱;检测波长360 nm;柱温25℃,流速:1.0 mL·min^-1。荭草苷、牡荆苷、牡荆苷-4′-O-葡萄糖苷和牡荆苷-2′-O-鼠李糖苷的平均回收率分别为98.5%、98.1%、99.1%和98.7%,相对标准偏差分别为0.8%、0.7%、0.8%和0.6%（n=5）。该方法分离效率高、分析速度快、检测灵敏度高、重复性好,可用于楠竹叶成分的检测分析。更多还原

【Abstract】 In this study,4 flavonoids in Phyllostachys pubescens extracts i.e.orientin,vitexin,vitexin-4’-O- glucoside,and vitexin-2’-O-rhamnose glycoside were simultaneously detected.The HPLC detected conditions were as follows:Kromasil-C18 column（5μm,4.6 mm×250 mm,Akzol Nobel,Sweden）,mobile phase was tetrahydrofuran:acetonitrile:methanol:0.5%aqueous acetic acid（10.3:1:0.7:88）,isocratic elution,the detection wavelength was 360 nm,column temperature was 25℃,and 1.0 mL·min^-1 flow velocity.The average recoveries of orientin,vitexin,vitexin-4’-O-glucoside,and vitexin-2’-O-rhamnose glycoside were 98.5%, 98.1%,99.1%,and 98.7%,respectively.The relative standard deviations were 0.8%,0.7%,0.8%and 0.6%（n = 5 ）,respectively.This HPLC method has been proved to be a method with high efficiency,rapid analysis,high sensitivity and good reproducibility for detection of flavonoids in P.pubescens extracts.更多还原