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外源NO对增补UV-B辐射下兴安落叶松幼苗叶片光合色素和叶绿素荧光特性的影响

Effect of Exogenous Nitric Oxide on Photosynthetic Pigments and Chlorophyll Fluorescence under Enhanced UV-B Radiation in Larix gmelinii

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【作者】 孟庆焕祖元刚郭晓瑞段喜华

【Author】 MENG Qing-Huan1,2 ZU Yuan-Gang1,2 GUO Xiao-Rui1,2 DUAN Xi-Hua1,2(1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin 150040;2.Engineering Research Center of Forest Bio-preparation,Ministry of Education,Northeast Forestry University,Harbin 150040)

【机构】 东北林业大学森林植物生态学教育部重点实验室东北林业大学林业生物制剂教育部工程研究中心

【摘要】 在增强UV-B辐射下,以3年生兴安落叶松幼苗为实验材料,研究了外源NO供体硝普钠(Sodium nitro-prusside,SNP)对幼苗的光合色素(Chla、Chlb和Car)和叶绿素荧光参数的影响。方差分析结果表明0.5 mmol.L-1的SNP对增补UV-B胁迫下的兴安落叶松幼苗产生显著影响。0.5 mmol.L-1的SNP能够显著抑制增补UV-B辐射后光合色素、Fv/Fm、ΦPSⅡ、Fv’/Fm’和qP的明显下降以及Chla/Chlb、Fo和NPQ的升高。表明了外源NO能够减轻UV-B辐射胁迫下兴安落叶松幼苗光合反应中心的生理损伤,从而增强兴安落叶松幼苗对增补UV-B辐射胁迫环境的适应能力。

【Abstract】 Under enhanced UV-B radiation,3-year-old seedlings of Larix gmelinii were chose as target to study the impact of exogenous nitric oxide on photosynthetic pigments(Chl a,Chl b and Car) and chlorophyll fluorescence.The results of variance analysis showed 0.5 mmol·L-1 sodium nitroprusside(SNP),a NO donor,have significant impact on nursery stocks of L.gmelinii under enhanced UV-B radiation stress.0.5 mmol·L-1 SNP can significantly inhibited the apparent decline in photosynthetic pigments,Fv/Fm,ΦPSⅡ,Fv′/Fm′ and qP,and the obvious increase in chl a/chl b,Fo and NPQ after supplement UV-B radiation.All these results indicated that exogenous nitric oxide could reduce the physical damage of photosynthetic reaction centers in seedlings of L.gmelinii under UV-B radiation stress,and enhancement of environmental adaptability of the seedling to supplementary UV-B radiation stress.

【基金】 黑龙江省自然科学基金申请项目(42400625-4)
  • 【文献出处】 植物研究 ,Bulletin of Botanical Research , 编辑部邮箱 ,2013年02期
  • 【分类号】S791.222
  • 【被引频次】4
  • 【下载频次】144
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