【摘要】 目的:检测转化生长因子-β1(TGF-β1)对瘢痕疙瘩成纤维细胞分泌基质金属蛋白酶-1(MMP-1)和金属蛋白酶组织抑制剂-1(TMP-1)表达的调节作用,探讨TGF-β1与瘢痕的关系。方法:培养的瘢痕疙瘩成纤维细胞经不同浓度的TGF-β1处理,采用酶联免疫法(ELISA)测定瘢痕疙瘩成纤维细胞上清液中MMP-1和TMP-1的水平。结果:瘢痕疙瘩中成纤维细胞MMP-1和TIMP-1的生成量显著高于正常皮肤(P<0.01);TGF-β1显著降低瘢痕成纤维细胞MMP-1的生成量(P<0.01),其作用在10~50 ng/mL呈剂量效应关系;不同浓度的TGF-β1对TIMP-1的生成量均无显著改变(P>0.05)。结论:TGF-β1在瘢痕生成过程中起重要作用。更多还原

【Abstract】 Objective:To investigate the regulatory effects of transforming growth factor- β1(TGF- β1) on the secretion of matrix metalloproteinase- 1(MMP- 1) and tissue inhibitor of metalloproteinase- 1(TIMP- 1) in human keloid- derived fibroblasts,and to understand the relationship between TGF-(31 and the scar formation.Method:The cultured keloid fibroblasts were treated with TGF- Bl at different concentrations.Enzyme linked immunosorbent assay(ELISA) method were performed to measure the expression of MMP- 1 and TIMP- 1 in human keloid fibroblasts supernatant.Results:The expression of MMP- 1 and TIMP- 1 was significantly higher in human keloid fibroblasts than in normal dermal fibroblasts(P < 0.01).The expression of MMP- 1 was decreased significandy after TGF- β1 was added into the cultured keloid fibroblasts(P < 0.01),with a dose- dependent as the increasing at concentrations of 10 ~ 50 ng/mL.However,the adding of TGF- β1 did not have any influence on the expression of TIMP-1(P > 0.05).Conclusion:TGF- β1 may play a role in the regulation of keloid formation.更多还原