【摘要】 目的推测新鉴定的神经肽S(NPS)经其受体激活下丘脑神经元参与下丘脑生理调节,运用神经功能活动形态定位法确定NPS在下丘脑靶神经元的分布。方法注射NPS(1 nmol,n=6)和生理盐水(n=6),c-fos免疫组化学分别标记大鼠中枢fos免疫反应神经元在下丘脑的分布,并统计分析fos免疫反应神经元数量在各核的变化。结果与生理盐水比较,NPS增加fos免疫反应神经元,数量分别为下丘脑视交叉上核322%,室旁核108%,背内侧核274%,腹内侧核126%,弓状核267%,穹窿周核520%,结节乳头体腹侧核641%,背侧核586%,外侧区378%(P<0.0001)。结论 NPS激活下丘脑上述靶神经元,可能与下丘脑睡眠觉醒周期、情绪、饮食、昼夜节律、温度和神经内分泌的调节功能有关。更多还原

【Abstract】 Objective To identify the distributions of neuropeptide S(NPS) induced neuronal activation in hypothalamus in rats because NPS,a newly identified neuropeptide,was presumed to activate the hypothalamic neurons through its receptors to participate hypothalamic physiological regulation.Methods Fos immunohistochemistry was employed to label the activated neurons following intracerebroventricular(i.c.v.) injection of NPS(1 nmol,n=6) and saline(n=6).The distribution and number of fos immunoreactive(-IR) neurons in the hypothalamus were observed, counted and statistically analyzed.Results The number of fos-IR neurons induced by NPS in the suprachiasmatic nucleus,paraventricular nucleus,dorsomedial hypothalamic nucleus,ventromedial hypothalamic nucleus,arcuate nucleus,perifornical nucleus,and the ventral and dorsal tuberomammillary nuclei and lateral hypothalamic area were respectively increased by 322%, 108%,274%,126%,267%,520%,641%,586%and 378%compared with saline(P<0.0001). Conclusion NPS-induced a large number of active neurons in the hypothalamus suggest that NPS is involved in hypothalamic physiological regulation including sleep-wake cycle,emotion, feeding,circadian rhythm,temperature and neuroendocrine.更多还原