【摘要】 目的:观察宁痫颗粒联合卡马西平对耐药性癫痫大鼠即早基因c-fos表达的影响,并探讨其作用机制。方法:Wistar雄性大鼠42只,按体质量随机分为假手术组,模型组,卡马西平+宁痫颗粒高[(0.03+4.80)g.kg-1.d-1]、中[(0.03+2.40)g.kg-1.d-1]、低剂量组[(0.03+1.20)g.kg-1.d-1],宁痫颗粒组(2.40 g.kg-1.d-1)和卡马西平组(0.03.g.kg-1.d-1),每组6只。采用海人酸海马CA3局部注射制作大鼠癫痫模型,术后第2天予苯妥英钠药物筛选14 d后制作耐药性癫痫模型。各组灌胃2周后免疫组织化学法检测大鼠即早基因c-fos表达的影响。结果:免疫组化实验显示:各治疗组大鼠耐药性癫痫模型颞叶皮层、海马CA3区c-fos阳性细胞较假手术组表达增强,密集分布,染色较深,同时又低于模型组;宁痫颗粒联合卡马西平各组均抑制c-fos蛋白的表达,作用优于卡马西平组(P<0.05)。各组间比较无显著差异性,宁痫颗粒组与卡马西平组无统计学差异。结论:宁痫颗粒联合卡马西平能抑制即早基因c-fos表达,两者联合疗效明显增强,以联合宁痫颗粒高剂量组疗效最佳,但治疗组指标均完全恢复到假手术组水平。更多还原

【Abstract】 Objective: To observe the Ningxian Particles combined with Carbamazepin(CBZ)on immediate early gene c-fos expression of drug-resistant epilepsy in rats,and to explore its mechanism.Methods: 42 male Wister rats were randomly divided into sham group,model group,Ningxian Particles combined with CBZ high[(0.03+4.80)g.kg-1.d-1],medium[(0.03+2.40)g.kg-1.d-1] and low dose groups [(0.03 +1.20)g.kg-1.d-1],Ningxian Particles group(2.40 g.kg-1.d-1)and CBZ group(0.03 g.kg-1.d-1)(n=6).Epilepsy model in rats was established by injection of kainic acid into hippocampal CA3.PHT drug screen was made 14 days after the production of drug-resistant epilepsy model.Immunohistochemical method in each group was given two weeks after the detection of the immediate early gene c-fos expression in rats.Results: Immunohisto-chemistry experiments: resistant epilepsy model temporal cortex of rats in each treatment group,increased expression of c-fos positive cells in hippocampal CA3 region compared to the sham group,and were densely distributed,deeply stained,while lower than that of the model group.Ningxian Particles combined with CBZ inhibited the expression of c-fos protein which was superior to that of CBZ group(P<0.05).No significant differences among groups,Ningxian particles group with CBZ group had no significant difference.Conclusion: Ningxian Particles combined with CBZ can inhibit the immediate early gene c-fos expression and significantly enhance combination of efficacy,the efficacy of high-dose group of the United Ningxian Particles treatment group indicators are fully restored to the sham-operated group level.更多还原