【摘要】 目的:采用HPLC法测定辽东楤木中楤木皂苷ⅹ的含量。方法:色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.05%磷酸溶液(35∶65);检测波长203 nm;柱温30℃;流速0.8 ml/min;进样量:10μl。结果:楤木皂苷ⅹ在0.60~4.80μg与峰面积具有良好的线性关系(r=0.9999),平均回收率98.14%,RSD为1.13%。结论:该方法灵敏、准确、重复性好,可用于辽东楤木的质量控制。更多还原

【Abstract】 Objective: To establish a method for determination of Araloside ⅹ in root bark of Aralia. Methods: HPLC was performed on a Kromasile C18 column(250 mm × 4.6 mm,5 μm) at 30 ℃.The composition of mobile phase was acetonitrile- 0.05% phosphoric acid(35∶65), the flow rate was 0.8 ml/min and the sample size was 10 μl. Results: The calibration curve showed good linearity over the range of 0.60 ~ 4.80 μg(r = 0.9999). The average recovery was 98.14% with RSD of 1.13%. Conclusion: This method is sensitive accurate, reproducible and it can be used for the quality control of root bark of Aralia.更多还原