【摘要】 目的:建立卵母细胞双电极电压钳记录技术。方法:建立双电极电压钳技术平台,并应用此平台,在去除滤泡膜的中国四川牛蛙卵母细胞上,记录内源性的外向离子电流。结果:卵母细胞孵育在正常ND96溶液中,将细胞膜钳制在-80 mV膜电位,给予一系列的去极化刺激可诱发出一个外向电流。在细胞外液中加入10 mM的TEA可阻断该电流80%以上,洗脱后电流幅值恢复至90%左右。将正常ND96溶液替换为无钙ND96溶液后,电流没有变化。结论:成功建立了卵母细胞双电极电压钳记录技术。去极化刺激在牛蛙卵母细胞上诱发出的外向电流为钾电流。更多还原

【Abstract】 Objective: To establish two-microelectrode voltage-clamp technique for oocytes. Methods: Twomicroelectrode voltage-clamp technique platform was established and the endogenous outward ionic currents were recorded on bullfrog oocytes after the removal of follicular theca. Results: As the oocytes were incubated in normal ND96 solution, the membrane potential was maintained at-80 mV, then depolarized to +50 mV in 10 mV increments, the outward cuttents were recorded. After applied 10 mmol/L TEA in the extracellular fluid, more than 80% currents were blocked, then recovery to about 90% after washout. The currents did not obviously change in ND96 solution without calcium. Conclusion: Two-microelectrode voltage-clamp technique is successfully established in oocytes. The outward currents induced by depolarizing stimulation on bullfrog oocytes were potassium currents.更多还原