节点文献
免疫亲和层析净化荧光光度法快速检测鸡饲料中黄曲霉毒素
Rapid detection of aflatoxins in chicken feed using immunoaffinity chromatography purification by fluorescence spectrophotometry
【摘要】 基于食品中采用免疫亲和层析净化荧光光度法测定黄曲霉毒素含量,建立了免疫亲和层析净化荧光光度法测定鸡饲料中黄曲霉毒素B1、B2、G1、G2含量。该方法的检出限2.0μg/kg,相对标准偏差低于10%,平均回收率85%~90%。
【Abstract】 A detection method of the total amount of aflatoxins(B1,B2,G1and G2)in chicken feed using immunoaffinity chromatography purification by fluorescence spectrophotometry was developed.The developed method exhibited a detection limit of 2.0μg/kg,the relative standard deviations below 10%and the recoveries of 85%~90%.
【关键词】 免疫亲和层析;
荧光光度法;
快速测定;
鸡饲料;
黄曲霉毒素;
【Key words】 immunoaffinity chromatography; fluorescence spectrophotometry; rapid detection; chicken feed; aflatoxin;
【Key words】 immunoaffinity chromatography; fluorescence spectrophotometry; rapid detection; chicken feed; aflatoxin;
【基金】 国家自然科学基金(海外及港澳学者合作研究基金-延续项目,31229004);中山市科技计划项目(2013)
- 【文献出处】 粮食与饲料工业 ,Cereal & Feed Industry , 编辑部邮箱 ,2013年11期
- 【分类号】S816.17
- 【被引频次】7
- 【下载频次】181