【摘要】 目的探讨非小细胞肺癌患者内科胸腔镜活检标本及胸腔积液中表皮生长因子受体(epidermal growth factor receptor,EGFR)的基因突变。方法收集非小细胞肺癌患者内科胸腔镜活检标本63例,同时选取该患者的胸腔积液标本63例,提取DNA后,采用ARAMS法同时进行EGFR19、21外显子突变检测。结果活检标本和胸腔积液标本中EGFR突变率分别为54.0%、50.8%,胸腔积液内的细胞EGFR基因突变率略低于活检组织标本,二者差异无统计学意义(P>0.05)。结论恶性胸腔积液可作为晚期非小细胞肺癌患者EGFR基因突变的检测标本。更多还原

【Abstract】 Purpose To study the EGFR gene mutations in the medical thoracoscopy biopsy tissue specimen and pleural effusion in advanced non small cell lung cancer patients. Methods Sixty-three cases of medical thoracoscopy biopsy tissue specimen and pleural effusion from advanced non small cell lung cancer patients were collected. Extraction of DNA from biopsy tissue specimen and pleural effusion was conducted,the biopsy tissue specimen and pleural effusion were analyzed for EGFR mutation in exons 19,21 by ARAMS.Results EGFR mutations were detected in 54. 0%(34 /63) of the biopsy tissue specimen and 50. 8%(32 /63) of pleural effusion.Pleural effusion mutations rate was slightly lower than that of biopsy,without statistically significant. Couclusion The pleural effusion fluid can be used to detect EGFR gene mutation.更多还原