【摘要】 目的探讨肝再生磷酸酶-2(phosphatase of regenerating liver-2,PRL-2)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及规律,并通过微血管密度(microvessel density,MVD)观察其表达与血管生成的关系。方法应用免疫组化SP法检测40例NSCLC组织及40例同一肺叶来源对应癌旁组织中PRL-2的表达,统计分析在不同临床病理参数之间的表达差异,通过CD34的表达计算MVD值,分析NSCLC组织中PRL-2的表达与肿瘤血管生成的相关性。结果 PRL-2在NSCLC组织中的阳性率为65%(26/40),其表达与患者年龄、性别、吸烟史及肿瘤的大小、病理类型等无关(P>0.05),在肿瘤的不同分化程度、淋巴结转移情况、TNM分期之间的表达差异有统计学意义(P<0.05),PRL-2在40例对应癌旁正常组织中均未发现阳性,同时分析表明NSCLC组织中PRL-2的表达与MVD值呈正相关(P<0.01)。结论与正常肺组织相比,PRL-2在NSCLC组织中存在高表达,其表达与肿瘤分化程度、淋巴结转移情况及TNM分期相关。与MVD相关,推断可能与肿瘤血管形成有关。更多还原

【Abstract】 Purpose To investigate the expression of phosphatase of regenerating liver-2(PRL-2) and its pattern in non-small cell lung cancer(NSCLC),and to observe the relationship of PRL-2 expression and angiogenesis.Methods The SP method of immunohistochemistry was used to detect the expression of PRL-2 in 40 cases NSCLC tissues and 40 cases of normal adjacent tissues from the same lung lobes,to analyze the differences of expression between different clinical and pathological parameters and to investigate the relationship of PRL-2 and tumor angiogenesis by calculating microvessel density(MVD) based on the expression of CD34.Results The positive expression rate of PRL-2 was 65% in NSCLC tissues(26/40),and it had no relationship with the age,sex,smoking history of patients,size and pathologic type of tumors(P>0.05),but the expression of PRL-2 was closely correlated with different degree of differentiation,lymph node metastasis and TNM stage of tumors(P<0.05).No positive expression of PRL-2 was found in 40 cases corresponding adjacent normal tissues.In addition,the analysis also showed that PRL-2 had positive correlation with MVD in NSCLC tissues(P<0.01).Conclusion Compared with normal lung tissues,PRL-2 is highly expressed in NSCLC tissues,which is correlated with the differentiation,lymph node metastasis,TNM stage and MVD,suggesting that the expression of PRL-2 may be associated with tumor angiogenesis.更多还原