【摘要】 目的探讨CC趋化因子受体7(CCR7)、L-selectin、CD44v6和MMP9在大肠癌组织中的表达及其与大肠癌淋巴转移的关系。方法采用免疫组化PV 9000两步法检测104例大肠癌组织(大肠癌组)、55例癌旁正常组织(癌旁组)和34例转移灶组织(转移组)中CCR7和L-selectin、CD44v6和MMP9的表达。结果大肠癌组、转移组中CCR7、L-selectin、CD44v6和MMP9阳性率明显高于癌旁组(P<0.05),有淋巴结转移者明显高于无转移者(P<0.05);CCR7与L-selectin、MMP9表达呈正相关(P<0.05);CD44v6与L-selectin、MMP9表达相关;L-selectin与MMP9的表达呈正相关。结论 CCR7、L-selectin、CD44v6和MMP9在大肠癌中的表达与大肠癌的发生、淋巴转移有关,它们可能共同参与了大肠癌发生及淋巴结转移过程。更多还原

【Abstract】 Purpose To investigate the expression of chemokine receptor 7(CCR7),adhesion molecule L-selectin,CD44v6 and MMP9 in human colorectal carcinoma,and analyze their correlation with lymph node metastasis.Methods The expression of CCR7,L-selectin,CD44v6 and MMP9 were tested by immunohistochemical method in 104 cases of colorectal carcinoma specimens(colorectal carcinoma group),55 cases of normal intestinal mucosa adjacent to carcinoma(adjacent group) and 34 cases of metastatic tumor tissue(metastasis group).Results The positive rate of CCR7,L-selectin,CD44v6 and MMP9 expression in the colorectal carcinoma group and metastatic tumor tissue were significantly higher than that in adjacent group and metastasis group(P<0.05).The expression of CCR7,L-selectin,CD44v6 and MMP9 in the tissue with lymph node metastasis was significantly higher than that in the tissue without lymph node metastasis(P<0.05).There was significantly positive correlation between expression of CCR7 and L-selectin,CCR7 and MMP9,CD44v6 and L-selectin,CD44v6 and MMP9,L-selectin and MMP9(P<0.05).Conclusions The expression of CCR7,L-selectin,CD44v6 and MMP9 are closely associated with development and metastasis of the colorectal carcinoma.They maybe participate together in the carcinogenesis and lymph node metastasis of colorectal carcinoma.更多还原