【摘要】 目的:观察电针对哮喘豚鼠气道重构模型气道病理结构的影响。方法:以10%卵清蛋白注射液腹腔注射致敏,第15 d起隔天以1%卵蛋白溶液超声雾化以激发(共6周)进行造模。采用电针背三针(大杼、风门、肺俞)干预治疗,隔天1次,共6周。治疗结束时,在光学显微镜下观察各组豚鼠肺组织和支气管的病理形态学改变、嗜酸粒细胞计数,并进行病理形态学测量。结果:模型对照组出现了哮喘气道重构的病理学改变,地塞米松治疗组和电针治疗组的肺组织也有不同程度的病变表现,但明显轻于模型对照组。模型对照组的支气管及肺组织中嗜酸性粒细胞数高于正常组(P<0.01),电针治疗组和地塞米松治疗组均低于模型对照组(P<0.01);与地塞米松治疗组相比较,电针治疗组嗜酸性粒细胞数略高(P<0.05)。模型对照组气道纤维组织厚度、气道内膜厚度高于正常组,电针治疗组和地塞米松治疗组都比模型对照组低(P<0.05);在小气道中,模型对照组气道平滑肌厚度高于正常组,电针治疗组和地塞米松治疗组均低于模型对照组(P<0.05);电针治疗组和地塞米松治疗组的观察指标无显著性差异(P>0.05)。结论:电针豚鼠背三针能降低气道重构豚鼠模型支气管及肺组织中嗜酸性粒细胞数,减轻气道炎症反应;降低豚鼠大气道的气道纤维组织厚度、气道内膜厚度及小气道平滑肌厚度。更多还原

【Abstract】 Objective:To observe the effect of electroacupuncture（EA） on airway pathological changes in asthmatic Guinea pigs with airway remodeling.Methods;The Guinea pig model was established by sensitization with intraperitoneal injection of 10%ovalbumin（OVA） in the first day and challenge with nebulized inhalation of 1% OVA solution in the 15^th day,every other day for 8 weeks.Every time before challenge,the interventive treatment of EA of back six points（Dazhu,Fengmen,Feishu） was carried out.After treatment,the pathological changes, eosinophils（EOS） count and morphometric analysis in bronchi and lung were observed under light microscope. Results;The pathological changes of airway remodeling occurred in the model group and the pathological changes in dexamethasone group and EA group were much relieved.The result of eosinophils（EOS） count showed that the number of EOS in the model group was much higher that in the normal group（P<0.01）,and decreased in dexamethasone group and EA group as compared with the model group（P<0.01）;as compared with that in dexamethasone group,EOS count in EA group was higher（P<0.05）.The results of morphometric analysis of bronchi and lung showed that the thickness of airway intima and thickness of fiber structure in main airway of the model group was higher than those in the normal group（P<0.05）,and were decreased in dexamethasone group and EA group as compared with the model group（P<0.05）.In small airway,the thickness of smooth muscle in the normal group was higher than those in the normal group（P<0.05）,and were decreased in dexamethasone group and EA group as compared with the model group（P<0.05）.The difference of the above three parameters was insignificant between dexamethasone group and EA group（P>0.05）.Conclusion;The interventive treatment of EA of back six points can reduce EOS count in bronchi and lung of asthmatic guinea pigs,relieve the inflammatory reaction,decrease the thickness of airway intima and thickness of fiber structure in main airway and the thickness of smooth muscle in small airway to relieve the damage of ultrastructure.更多还原