【摘要】 目的探讨转录因子Kruppel样因子(KLF)4和KLF5在乳腺浸润性导管癌中的表达,分析其表达与乳腺浸润性导管癌的发生、发展及临床病理因素之间的关系。方法采用免疫组化SP法检测30例乳腺浸润性导管癌组织及10例对应癌旁正常组织中KLF4和KLF5蛋白的表达,分析其表达与乳腺癌临床病理特征的关系。结果 KLF4和KLF5均主要表达于细胞核。乳腺浸润性导管癌组织中KLF4低表达,阳性表达率为6.7%,低于癌旁正常乳腺组织(70.0%),差异有统计学意义(P<0.05);KLF5在乳腺癌组织中高表达,阳性表达率为90.0%,高于癌旁正常乳腺组织(30.0%),差异有统计学意义(P<0.05)。乳腺浸润性导管癌组织中KLF4和KLF5的表达与年龄、绝经情况、组织学分级、淋巴结转移、ER及PR表达无关(P>0.05),而KLF5与HER-2表达水平有关(P<0.05)。KLF4和KLF5在乳腺浸润性导管癌中的表达无相关性(r=-0.356,P=0.053)。结论 KLF4和KLF5表达与乳腺浸润性导管癌的发生密切相关,可以作为预测乳腺浸润性导管癌发生、发展及转移的评价指标之一。更多还原

【Abstract】 Objective To investigate the expression levels of Kruppel-like factor( KLF) 4 and KLF5 and the relationship between their expressions and clinicopathological characteristics in breast invasive ductal carcinoma. Methods Thirty cases of surgical removal of breast cancer tissue diagnosed with invasive ductal carcinoma by postoperative paraffin pathology and 10 cases of normal breast tissue adjacent to carcinoma were collected from March 2012 to October 2012 in the Affiliated Hospital of Hebei University. The immunohistochemical staining was performed to detect KLF4 and KLF5 levels in invasive ductal carcinoma or adjacent normal breast tissues. The correlation between their expressions and clinicopathological characteristics were analyzed. Results Immunohistochemical results showed both of KLF4 and KLF5 mainly expressed in the nucleus. The KLF4 expression level in invasive ductal carcinoma was significantly lower than that in adjacent normal breast tissues( 6. 7% vs. 70. 0%,P < 0. 05). The KLF5 expression level in invasive ductal carcinoma was significantly higher than that in adjacent normal breast tissues( 90. 0% vs. 30. 0%,P < 0. 05). The positive expression rates of KLF4 and KLF5 were not significantly associated with patients’ age,menopausal status,histological grade, lymph node metastasis,ER and PR expression levels( P > 0. 05). The KLF5 expression level in breast cancer was correlated with HER-2 expression level( P < 0. 05). Moreover,no correlation was found between KLF4 and KLF5 expression in invasive ductal carcinoma( r =- 0. 356,P = 0. 053). Conclusion The KLF4 expression level decreases and KLF5 expression level increases in invasive ductal carcinoma,suggesting their important roles of KLFs in breast cancer.更多还原