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自身对照在交叉配血不合分析中的应用
Application of self-control test in causes analysis of incompalible cross matching
【摘要】 目的:分析在交叉配血不合时平行自身对照实验的意义,指导筛检出相合的血液。方法:利用微柱凝胶卡对交叉配血不合的患者进行直接抗人球蛋白试验、抗体筛检试验、自身对照试验,必要时使用谱细胞进行抗体特异性的鉴定和RH分型试验,根据各项结果判断是否有完全相合的血液及如何筛检相合的血液。结果:在交叉配血不合的患者中,抗体筛检阳性占93.94%,部分自身对照阳性的患者有不经处理就完全相合的血液。结论:在交叉配血不合的患者中,抗体筛检实验要引起足够的重视,自身对照阳性的患者要通过实验鉴定其抗体的特异性,以保证输血安全。
【Abstract】 Objective:To analyse the significance of the self-control test in incompatible cross matching in parallel and to guide to screen the matched blood.Method:The patients with incompatible cross matching were carried out direct antiglobulin test(DAT),antibody screening test and self-control test using micro column gel.The specific antibodies with the celllineages and RH subgroups were identified when necessary.To determine whether there is a completely matched blood and how to screen the compatible blood by the results of experiments.Result:In patients with incompatible cross matching,the positive results of antibody screening were 93.94%.The completely matched blood were discovered in partial patients with the positive self-control tests.Conclusion:Antibody screening test is very important in patients with incompatible cross matching.In order to ensure the safety of blood transfusion,the specificity of the antibodies should be identified in the patients with the positive self-control tests.
【Key words】 cross matching; self-control test; antibody screening test; DAT;
- 【文献出处】 临床血液学杂志 ,Journal of Clinical Hematology , 编辑部邮箱 ,2013年08期
- 【分类号】R457.1
- 【下载频次】22