【摘要】 目的:探讨宁夏回、汉族人群脂联素基因SNP+276G/T单核苷酸多态性与冠心病的相关性。方法:收集回、汉族冠心病患者95例和93例以及回、汉族健康体检者(对照组)98例和102例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测SNP+276G/T单核苷酸多态性,酶联免疫吸附法(ELISA)检测血清脂联素浓度。结果:回、汉族人群脂联素基因SNP+276G/T多态GG、TG、TT基因型分布频率冠心病组分别为0.137、0.789、0.074和0.581、0.355、0.064;对照组分别为0.316、0.531、0.153和0.598、0.373、0.029。G及T等位基因分布频率冠心病组分别为0.532、0.468和0.242、0.758;对照组分别为0.582、0.418和0.216、0.784。脂联素基因SNP+276G/T多态GG,TG,TT基因型分布频率在回族冠心病组及对照组间差异有统计学意义(P<0.05);G及T等位基因分布在2个民族冠心病组与对照组比较差异均无统计学意义(P>0.05)。宁夏回、汉族冠心病组血清脂联素水平均低于对照组(P<0.05),回、汉族人群TT+TG基因型血清脂联素水平与GG型相比均无显著差异(P>0.05)。Logistic回归分析结果显示,回族人群中携带TT+TG基因型者发生冠心病的风险增加(OR=5.814,95%CI:3.332～10.144)。结论:脂联素基因SNP+276G/T多态性可能与宁夏回族冠心病相关,与宁夏汉族冠心病无关。更多还原

【Abstract】 Objective:To investigate relationship between adiponectin gene +276 nucleotide G/T polymorphism with coronary heart disease(CHD)in Hui and Han nationalities in Ningxia.Method:The study was performed in 95 Hui patients and 93 Han patients with CHD and was performed in 98 Hui healthy peoples and 102 Han healthy peoples(NC group).Polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP)and enzyme-linked immunosorbent assay(ELISA)were used to detect the gene polymorphism of adiponectin and the serum adiponectin level.Result:The frequencies of 276G/T genotypes of GG,TG,and TT were 0.137,0.789 and 0.074in Hui CHD patients and 0.316,0.531and 0.153in NC group;0.581,0.355and 0.064in Han CHD patients and 0.598,0.373and 0.029 in NC group respectively.The frequencies of G and T were 0.532and0.468,and 0.242and 0.758in CHD group;0.582and 0.418,and 0.216 and 0.784in NC group.There was significant difference in genotypes between CHD group and NC group in Hui nationality(P<0.05).There was no significant difference in allele frequencies in Hui and Han nationalities(P>0.05).Compared with the NC group,the serum adiponectin level of CHD group was lower than those of NC group in both Hui and Han nationalities(P<0.05).No matter Hui or Han nationality,there was no significant difference of serum adiponectin level between TG+GG genotype and TT genotype(P>0.05).Logistic regression analysis showed that those carrying TT+TG genotype had an increased risk of CHD in Hui population(OR=5.814,95%CI:3.332-10.144).Conclusion:+276 nucleotide G/T polymorphism of adiponectin gene might be associated with CHD in Hui population but not in Han population in Ningxia.更多还原