【摘要】 目的 探讨Bcl-2/Bax及Caspase-3在心肌缺血再灌注及后处理对大鼠肺损伤的表达及作用。方法 雄性SD大鼠30只随机分为3组,每组10只,假手术组(S)、心肌缺血/再灌注组(1R)、缺血后处理组(IPost)。结扎左冠状动脉前降支制备心肌缺血/再灌注模型,后处理组于再灌注前1 min内,连续3个循环灌注10 s,缺血10 s,再灌注120 min后快速取肺。SP法测定肺组织内Bcl-2/Bax及Caspase-3。TUNEL法检测细胞凋亡。结果 与S组相比较,IR组,IPost组,Bax,Caspase-3,Bcl-2表达减少(P<0.01),细胞凋亡增多;与IR组相比较,IPost组,Bax,Caspase-3,细胞凋亡(TUNEL)表达减少,Bcl-2表达增多(P<0.01),细胞凋亡减少。结论 缺血后处理可以诱导Bcl-2表达增强,Bax及Caspase-3表达降低,从而减轻肺损伤。更多还原

【Abstract】 Objective To observe the expression and significance of Bcl-2/Bax and Caspase-3 in acute lung injury induced by myocardial ischemia-reperfusion(IR)and ischemic post- conditioning.Methods Thirty SD rats were randomly allocated into the sham-operated group(group S),the myocardial ischemia-reperfusion group(IR group) and the ischemic post-conditioning group(group IPost).The model of myocardial IR was established by the occlusion of anterior descending branch of left coronary artery.Rats in the group IPost received 3 cycles of 10 s reperfusion followed by 10 s ischemia within the first minute before the reperfusion.The lung was immediately removed after 120 min of reperfusion.The SP method was applied for the test of and apoptosis was detected by the TUNEL assay.Results Compared with group S, the expression of Bcl-2/Bax and Caspase-3 in the IR and IPost group were significantly reduced with the increasing of apoptotic cells.In the IPosl group,the expression of Bax,Caspase-3 and TUNEL outcomes were reduced and the Bcl-2 increased compared with the IR group.This indicated the reduction of apoptosis.Conclusion Ischemic post-conditioning increases the Bcl-2 but decreases the expression of Bax and Caspase-3,which lessen acute lung injury.更多还原