【摘要】 目的研究N-甲基-D-天冬氨酸(NMDA)受体拮抗剂MK-801对新生7 d大鼠室管膜下区(SVZ)胶质纤维酸性蛋白(GFAP)表达的影响。方法将40只新生SD大鼠分成对照组和MK-801组,各组按出生后(P)时间点再随机分成4个亚组:P7 d、P14 d、P21 d、P28 d组。新生大鼠均于生后第3天给药,MK-801组腹腔注射MK-801 10 mg.kg-1;对照组腹腔注射同量生理盐水。通过免疫组化学方法观察大鼠SVZ区GFAP阳性细胞数。结果①对照组GFAP阳性细胞数于P14 d开始增加,至P21 d达最大值;但P28 d时阳性细胞迅速下降;②MK-801组GFAP阳性细胞数与对照组相比,P7 d和P28 d无明显差异,P14 d(65.40±6.11)和P21 d(239.60±12.92)细胞数明显减少;而对照组P14 d(79.20±5.26)、P21 d(265.20±7.40)GFAP阳性细胞数明显增多,差异有显著统计学意义(P<0.01)。结论 NMDA受体拮抗剂MK-801对正常新生大鼠SVZ区GFAP的表达有抑制作用,能够抑制SVZ区神经干细胞的增殖和分化。更多还原

【Abstract】 Aim To investigate the effects of the N-methyl-D-aspartate(NMDA)-receptor antagonist MK-801 on the expression of glial fibrillary acidic protein(GFAP) in the subventricular zone(SVZ) of neonatal rat. Methods 40 neonatal SD rats were assigned into two groups, a MK-801 group and a control group. Each group was respectively divided into four time points, including P7 d (postnatal 7 days), P14 d, P21 d and P28 d. Rats in the experiment group were intraperitoneally injected selective non-competitive NMDA receptor antagonist MK-801 (10 mg·g-1), while rats in the control group were intraperitoneally injected saline. Immunohistochemical staining was used to assay the number of GFAP-positive cells in the SVZ. Results ①In the control group, the number of GFAP-positive cells increased at P14 d, and the number of GFAP-positive cells reached maximum at P21 d, positive cells decreased significantly at 28 d. ②Compared with the control group, the number of GFAP-positive cells reduced significantly at P14 d (65.40±6.11) and P21 d (239.60±12.92), and the number of GFAP-positive cells increased significantly [P14 d(79.20±5.26), P21 d(265.20±7.40)](P<0.01), while the number of positive cells without obvious changes at P7 d and P28 d in the MK-801 group. Conclusion NMDA-receptor antagonist MK-801 could reduce the expression of GFAP in the SVZ, and inhibit the neural stem cells proliferation and differentiation.更多还原