【摘要】 目的探讨不同浓度瑞芬太尼对乳鼠离体脑海马神经细胞存活及凋亡的影响。方法原代培养乳鼠脑海马神经细胞:取出生7d的新生乳鼠海马神经细胞,原代培养7d,随机分为正常对照组(G组);瑞芬太尼72ng/ml处理组(R1组);瑞芬太尼126ng/ml处理组(R2组);瑞芬太尼180ng/ml处理组(R3组)。采用MTT检测脑海马神经细胞存活率、凋亡率,在电镜下观察海马神经细胞超微结构的变化。结果与G组相比较,R1、R2、R3组神经细胞存活率均降低(P<0.05),其中R2、R3组神经细胞存活率显著降低(P<0.01);R1、R2、R3组内比较海马神经细胞存活率随瑞芬太尼浓度的升高而降低(P<0.05或P<0.01)。与G组比较,R1、R2、R3组海马神经细胞凋亡率明显增加(P<0.05),其中R2、R3组神经细胞凋亡率显著增加(P<0.01);R1、R2、R3组内比较海马神经细胞凋亡率随瑞芬太尼浓度的升高而增加(P<0.05或P<0.01)。G组胞浆内细胞器结构正常。R1、R2、R3组可见不同程度的神经细胞损伤,细胞器相互靠近,其中R2、R3组神经细胞损伤程度更重,出现空泡样变性(空穴现象)。结论在本研究的浓度范围内,瑞芬太尼可抑制新生乳鼠海马神经细胞的存活并促进其凋亡,并且浓度越高,抑制其存活及促进其凋亡的作用越明显。更多还原

【Abstract】 Objective To investigate the effect of remifentanil with different concentrations on the cell viability and apoptosis in primary cultured hippocampal neurons of neonatal rat.Methods For the primary culture hippocampal neurons in neonatal rat,primary culture hippocampal neurons isolated from newborn SD rats(7days)and cultured 7days and then randomly divided into 4groups:group control(group G),group remifentanil 72ng/ml(group R1),group remifentanil 126ng/ml(group R2),group remifentanil 180 ng/ml(group R3). The hippocampal neurons cell histopathological changes were observed with electron microscopy.The apoptosis were measured by flow cytometry and the survival of these cells were determined using MTT[3-(4,5-two methylic thiazole-2)-2,5-two phenyltetrazole bromide salts].Results The neuronal viability was lower(P<0.05)in the groups R1,R2,and R3 when compared to the group G,and there was significantly difference in the parameter mentioned above between groups R2and R3(P<0.01).The apoptosis rate of hippocampal neurons was higher(P<0.05)in the groups R1,R2,R3than in group G,and there was significantly difference in the parameter mentioned above between groups R2and R3(P<0.01).Regards the histopathological changes of neurons cells,the mass of organelles structure was normal in group G,however,different degrees damage of nerve cell and organelles near each other were appeared in groups R1,R2,R3after drug treatment.The hippocampal neurons changes were even worse and appear empty bubble sample degeneration(cavitation)in groups R2,R3.Conclusion Remifentanil can inhibit the cell survival and promote apoptosis of neonatal rat hippocampal neurons in the study within the scope of concentration.It was positive related to the concentration.更多还原