【摘要】 目的:探讨生长分化因子-9(growth differentiation factor-9,GDF-9)在人膀胱癌组织中的表达及其与膀胱癌病理分级和临床分期的关系。方法:运用免疫组化技术检测GDF-9在50例不同分期分级的膀胱癌组织和15例正常膀胱组织之间的蛋白表达情况。结果:①正常人膀胱组织中GDF-9呈高表达,且GDF-9蛋白主要定位于膀胱移行上皮细胞的胞浆中。而膀胱癌组织中GDF-9呈低表达或不表达。②GDF-9阳性表达率在膀胱癌病理分级间分别:Ⅰ级47.6%(10/21),Ⅱ级26.7%(4/15),Ⅲ级7.1%(1/14),差异具有统计学意义(P<0.05)。GDF-9阳性表达率在膀胱癌临床分期间分别:Ta～T1期40.6%(13/32),T2～T3期11.1%(2/18),差异具有统计学意义(P<0.05)。结论:GDF-9在膀胱癌组织中低表达或不表达,且与膀胱癌病理分级和临床分期呈负相关。GDF-9可能是一个潜在的人膀胱肿瘤抑癌基因。更多还原

【Abstract】 Objective:To investigate the expression of growth differentiation factor-9(GDF-9)in bladder transitional cell carcinoma(BTCC)and its clinical significance in the malignancy of BTCC.Method:Immunohistochemical methods was used to detect GDF-9 protein in 50 cases of BTCC and 15 cases of normal bladder muscosa served as the controls.Result:In normal bladder tissues,stronger staining of GDF-9 was seen in transitional cells,both in the cytoplasm and in the nucleus.In contrast,the staining of GDF-9was notably weak or absent in cancer cells of tumour tissues.The positive expression rate of GDF-9 in GradeⅠ,Ⅱ and Ⅲ according to pathological grade of BTCC was 47.6%(10/21),26.7%(4/15) and 7.1%(1/14),respectively.There were significant differences among the three pathological grades.The positive expression rate of GDF-9 in Stage Ta-T1and T2-T3according to clinical stages of BTCC was 40.6%(13/32)and 11.1%(2/18),respectively.There were significant differences between the two clinical stages.Conclusion:GDF-9 is expressed at lower levels in human bladder cancer tissues compared with normal transitional cells of the bladder.GDF-9 levels are inversely correlated with the pathological grade and clinical stages of bladder cancer.It suggests that GDF-9 is a potential tumour suppressor in human bladder cancer.更多还原