【摘要】 目的探讨梗阻性黄疸兔血清心肌标志物含量变化与梗阻性黄疸患者血清心肌标志物含量变化的差别。方法用止血钳钳夹实验兔胆总管以造成可逆型梗阻性黄疸,以Beckman全自动免疫分析仪测定其血清心肌标志物;同批测定结石性梗阻性黄疸患者血清心肌标志物。对测定数据进行统计分析和比较。结果梗阻黄疸实验兔(梗阻30 d)TBil、DBil显著高于对照组(P<0.01);其血清肌酸激酯同工酶(CK-MB)、肌红蛋白(MYO)、肌钙蛋白Ⅰ(TROPI)分别为(8.76±2.36)、(88.4±24.9)、(0.35±0.11)μg/L比对照组(2.36±1.12)、(31.3±12.4)、(0.01±0.01)μg/L有显著增加(P<0.01)。梗阻性黄疸患者治疗前血清CK-MB、MYO、TROPI分别为(23.93±5.11)、(188.8±36.5)、(0.34±0.08)μg/L,显著高于健康对照组(2.06±0.82)、(25.4±10.2)、(0.01±0.01)μg/L,P<0.01)。结论实验性梗阻性黄疸兔血清心肌标志物含量与人类梗阻性黄疸患者一样显著增加,人或实验动物梗阻性黄疸都可造成心肌损害。更多还原

【Abstract】 Objective To investigate whether the serum markers of cardiac function determined in a rabbit model with experimentally induced obstructive jaundice are similar to those in human patients with obstructive jaundice.Methods Reversible obstructive jaundice was induced in rabbits by gripping the general bile ducts with a 15 cm hemostasis pincer for 3 min.Sham-operated rabbits were used as controls.Serum samples were taken at day 30 post-surgery.Thirty patients with obstructive jaundice due to biliary stones,and 30 age-and sex-matched healthy controls were recruited for study.Fasting serum samples were collected for analysis.Changes in serum cardiac markers were assayed by the Beckman-Coulter CX-7 automatic biochemical analyzer and 81600N immunoassay system.Significance of differences between groups was assessed by Dunnett’s t-test.Results In the rabbit model,total bilirubin(TBil) and direct bilirubin(DBil) were significantly elevated(vs.sham-operated control,P< 0.01).In addition,the jaundice model showed significantly increased serum creatine kinase isoenzyme(CK-MB;8.76±2.36 vs.2.36±1.12 μg/L),myoglobin(MYO;88.4±24.9 vs.31.3±12.4 μg/L),and cardiac troponin-I(TROPI;0.35±0.11 vs.0.01±0.01 μg/L)(all P< 0.01).Similarly,the patients with obstructive jaundice showed significantly higher levels of CK-MB(23.93±5.11 vs.2.06±0.82 μg/L),MYO(188.8±36.5 vs.25.4±10.2 μg/L),and TROPI(0.34±0.08 vs.0.01±0.01 μg/L)(all P< 0.01).Conclusion The elevated serum levels of cardiac markers in the rabbit model of experimentally induced obstructive jaundice rabbits are resemble those in human patients with obstructive jaundice due to biliary stones.The rabbit model is an appropriate tool for investigating myocardial injury related to obstructive jaundice.更多还原