【摘要】 目的了解消化液处理标本抗酸染色在提高结核分枝杆菌检出率方面的价值。方法以BACTEC MGIT 960检出阳性样本为参照,消化液处理标本抗酸染色比较直接涂片抗酸染色和L-J培养这两种方法,对消化液处理标本抗酸染色的阳性检出率做出评价。结果 MGIT 960检出的阳性样本共635例,经消化液处理直接涂片的阳性检出率为48.03%(305/635),与直接涂片抗酸染色阳性检出率(62.20%(395/635))比较,两者无统计学差异;较L-J培养管87.55%(556/635)的阳性检出率,两者有显著的统计学差异。结论消化液处理后的抗酸涂片并不比多次直接涂片抗酸染色对结核分枝杆菌的阳性检出率高。更多还原

【Abstract】 Objective To evaluate the acid-fast staining of digestive juice processed specimen in improving the detection rate of mycobacterium tuberculosis.Methods Taking the positive samples detected by BACTEC MGIT 960 as a reference,the acid-fast stainingof digestive juice processed specimen was compared to the direct smear and the L-J cultured method,and then the detection results of positive rate were evaluated.Results 635 positive samples were detected by the MGIT 960.The positive detective rate of digestive juice processed samples was 48.03%(305/635),62.20% of direct smear(395/635),and 87.55% of L-J cultured method(556/635).There was no significant difference of positive detective rate between the digestive juice processed samples and direct smear(X2=136.327,P=0.000),but it showed a significant difference between the digestive juice processed samples and L-J cultured method(X2=23.039,P=0.000).Conclusion The acid-fast staining of digestive juice processed specimen has no advantage to direct smear and L-J cultured method.The acid-fast staining of direct smear is a simple and rapid method in the discovery of tuberculosis,and the L-J cultured method has a relatively higher positive detective rate though it takes a little longer time.更多还原