【摘要】 目的:探讨双氢青蒿素(DHA)对鼻咽癌CNE-2细胞的生长抑制作用及其机制。方法:以体外培养的CNE-2细胞为研究对象,分别采用CCK-8法观察不同浓度和时间DHA对CNE-2细胞增殖的抑制作用,流式细胞仪Annexin V-FITC法检测CNE-2细胞的凋亡率,caspase-3活性检测法检测不同浓度DHA处理后CNE-2细胞中caspase-3的活性变化。结果:CCK-8法显示,与对照组相比,随DHA浓度的增加,CNE-2细胞的增殖显著抑制(P<0.05);流式细胞仪Annexin V-FITC法,结果显示DHA诱导CNE-2细胞凋亡具有浓度依赖性;caspase-3活性检测法显示,与对照组相比,随DHA浓度的增加,caspase-3的活性显著提高(P<0.05)。结论:DHA能有效抑制CNE-2细胞的增殖并诱导其凋亡,其机制可能是能上调CNE-2细胞中caspase-3的活性。更多还原

【Abstract】 Objective:Dihydroartemisinin is a traditional anti-malarial drug,a derivative of the artemisinin,it has anti-tumor activity of a variety of tumor cells.This study investigated the effect of growth inhibition of nasopharyngeal carcinoma cells line CNE-2 induced by dihydroartemisinin and its possible mechanism.Method:The effect of DHA on the cell proliferation of CNE-2 was detected by CCK-8 assay with different concentrations and time.The effects of DHA on the cell apoptosis of CNE-2 were detected by Annexin V-FITC assay through flow cytometry and caspase-3 activity assay.Result:CCK-8 experimental results show that CNE-2 cell proliferation was suppressed with DHA treatment,as compared with the control group.DHA could induce marked apoptosis in CNE-2 by apoptosis assay,as compared with the control group,The percentages of apoptotic cells increased along with the increase of DHA concentrations in CNE-2;The activity of caspase-3 was increased following DHA treatment in a dose-dependent manner;Conclusion:DHA could effectively inhibit proliferation and induce apoptosis of human nasopharyngeal carcinoma cells line CNE-2,the possible mechanism DHA induce apoptosis of CNE-2 cells by upregulating the expression of caspase-3.更多还原