【摘要】 目的比较3种脂肪乳剂对脂多糖(LPS)诱导的急性肺损伤大鼠(ALI)IL-1β表达及肺泡上皮细胞凋亡的影响。方法 100只SD幼年大鼠按随机数字分为Blank组(生理盐水+生理盐水)、LPS组(生理盐水+LPS)、Intra组(20%脂肪乳Intralipid+LPS)、Clino组(20%脂肪乳Clinoleic+LPS)和Omega组(10%脂肪乳Omegaven+LPS)。各组分别采用生理盐水或脂肪乳剂处理7 d;并均在取标本前8 h于气管内滴入生理盐水或脂多糖,建立ALI大鼠模型;观察各组大鼠肺组织的病理改变,检测肺泡上皮细胞凋亡,并计算凋亡指数(AI),测定IL-1βmRNA变化及蛋白浓度。结果与Intra组相比,Clino组和Omega组IL-1βmRNA表达及IL-1β水平较低(P均<0.005),而Clino组和Omega组IL-1βmRNA浓度及蛋白水平差异无统计学意义(P>0.005)。各组大鼠肺组织均可见明显炎症细胞浸润和出血。Clino组和Omega组凋亡系数(AI)均低于LPS组和Intra组(P均<0.005);Clino组和Omega组AI值差异无统计学意义(P>0.005)。结论 Clinoleic和Omegaven可能通过降低IL-1β而减轻ALI大鼠炎症反应,并减少肺泡上皮细胞凋亡。更多还原

【Abstract】 Objectives To compare the effects of three kinds of lipid emulsions on IL-1β expression and alveolar epithelial cell apoptosis in LPS-induced acute lung injury(ALI) in rat.Methods One hundred young Sprague-Dawley rats were divided into control group,LPS group,Intra group(20% Intralipid),Clino group(20% Clinoleic) and Omega group(10% Omegaven).Each group was treated with normal saline or different lipid emulsions for seven days.The rats were intratracheally instilled with normal saline or LPS to establish the rat model of ALI 8 hours before sacrifice.The pathologic changes in lung tissue section were observed and the apoptosis index(AI) was measured.The expression level of IL-1β mRNA was determined by RT-PCR.Meanwhile the concentration of IL-1β in broncho-alveolar lavage fluid(BALF) was measured by enzyme-linked immunospecific assay(ELISA).Results The expression of IL-1β mRNA and the level of IL-1β in BALF in Intra group were significantly elevated as compared to Clino group and Omega group(both P<0.005),but there was no significant difference(P>0.005) between Clino group and Omega group.HE-stained lung tissues showed obvious infiltration of inflammatory cells and bleeding in all groups.The AI was lower in Clino group and Omega group than in LPS group and Intra group(both P<0.005) and the difference was not significant between Clino group and Omega group(P>0.005).Conclusions Clinoleic and Omegaven may relieve inflammation by down-regulating IL-1β and reduce the alveolar epithelial cell apoptosis in rats with acute lung injury.更多还原