【摘要】 目的:通过对小鼠腹腔注射D-gal制造衰老模型小鼠。方法:用藏灵菇发酵乳对小鼠进行灌胃处理,采用黄嘌呤氧化酶法,TBA反应比色法,测定小鼠脑组织中总超氧化物歧化酶(T-SOD),谷胱甘肽过氧化物酶(GSH-Px)活性,丙二醛(MDA)的含量变化,进一步研究藏灵菇发酵乳的抗氧化抗衰老作用及其机理。结果:发现藏灵菇发酵乳能极显著提高D-半乳糖所致衰老小鼠脑组织T-SOD活性,显著提高GSH-Px活性,极显著降低MDA的含量。结论:证明藏灵菇发酵乳具有显著清除衰老小鼠脑组织氧自由基能力,提高抗氧化酶活性,抑制小鼠脑自由基从而发挥抗衰老作用。更多还原

【Abstract】 Objective: Through the establishment of D-gal induced subacute senile mouse model.Methats: Given Tibetan Kefir fermented milk gastric lavage treatment,to determine the total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px) activity,malondialdehyde(MDA) content changes in brain tissue of mice,and further study antioxidant effect and its mechanism of Tibetan Kefir fermentation milk.Results: The results showed that Tibetan Kefir of fermented milk can significantly improve D-galactose induced aging mice brain tissue T-SOD activity,significantly increase the GSH-Px activity,decreased significantly the content of MDA.Conclusion: It has been proved that Tibetan Kefir fermented milk has a significant effect on scavenging oxygen free radicals in brain tissue of aging mice,improving the antioxidant enzyme activity,and inhibiting mouse brain free radical so as to play the anti-aging role.更多还原