【摘要】 目的:观察YAP2蛋白在涎腺腺样囊性癌组织及细胞中的表达。方法:通过免疫组织化学法检测YAP2蛋白在涎腺腺样囊性癌组织及细胞中的表达,用Western印迹法检测表皮生长因子受体(epidermal growth factor receptor,EGFR)对涎腺腺样囊性癌(salivary gland adenoid cystic carcinoma,SACC)SACC-83细胞YAP2蛋白的抑制。结果 :SACC组织中YAP2蛋白在肿瘤细胞胞质中阳性表达,正常腺体中YAP2蛋白主要表达在部分导管上皮中,两组相比差异有统计学意义(P<0.05)。免疫细胞化学染色结果显示SACC-83中YAP2蛋白阳性表达,加入EGFR蛋白后,SACC-83中YAP2蛋白表达有所降低。Western印迹法结果显示,不同质量浓度的EGFR蛋白(5、10、20、40μg/L)对SACC-83细胞YAP2蛋白的表达都有一定抑制作用,其中40μg/L质量浓度组抑制作用最明显。结论:YAP2蛋白在涎腺腺样囊性癌组织及细胞中高表达,EGFR蛋白对YAP2蛋白在SACC-83中表达有抑制作用。更多还原

【Abstract】 Objective:This study aimed to observe the expression of YAP2 in salivary gland adenoid cystic carcinoma tissue and cell line.Methods:YAP2 protein in salivary gland adenoid cystic carcinoma tissue and cells were identified by immunohistochemistry with a multiclonal antibody.EGFR inhibition of YAP2 in SACC-83 cells was detected by western blotting.Results:In SACC,positive expression of YAP2 were in the cytoplasm.In normal salivary gland,YAP2 positive were identified in some duct epithelia.There was statistical significance between them(P <0.05).Expression of YAP2protein in SACC-83 was down-regulated by the addition of EGFR.Western blot analysis showed EGFR(5,10,20,40 μg/L)inhibited the expression of YAP2 in SACC-83,and 40 μg/L was most effective.Conclusion:YAP2 protein is overexpressed in salivary gland adenoid cystic carcinoma tissue and cells.EGFR protein inhibits the expression of YAP2in the SACC-83.更多还原