【摘要】 目的:观察橙皮苷对急性血瘀模型大鼠血液流变学指标的影响。方法:大鼠按分组分别ig给予橙皮苷橙皮苷80、160和320mg·kg-1及阿司匹林100mg·kg-1。每日早晚ig给药各1次,共7次。第5次给药后,sc给予肾上腺素加冰浴造成急性血瘀模型。锥板法测定全血粘度和血浆粘度;微量毛细血管法测定血细胞比容(Hct);光电比浊法测定二磷酸腺苷(ADP)诱导的血小板聚集;光电电磁法测定凝血参数。结果:与正常对照组相比,模型组大鼠全血粘度和血浆粘度升高,红细胞聚集指数和血小板最大聚集率显著增加。Hct显著升高,纤维蛋白原(Fib)含量显著增加,凝血酶时间(TT)、活化部分凝血活酶时间和凝血酶原时间(PT)均显著缩短(P<0.01)。与模型组相比,橙皮苷160和320mg/kg能显著降低全血粘度及血浆粘度,明显降低Hct、红细胞聚集指数和血小板最大聚集率、显著延长PT,橙皮苷160和320mg/kg显著降低Fib含量,延长TT。结论:橙皮苷能显著改善血瘀大鼠血液流变学异常。更多还原

【Abstract】 AIM To evaluate effectc of hesperidin on the hemorheological abnormality in rats with acute blood stasis. METHODS The rats were randomly divided into normal control,model,aspirine 100mg/kg,hesperidin 80、160 and 320mg/kg groups.The rats were ig given hesperidin or aspirin twice a day, altogether 7 times. After the 5th administration, the acute blood stasis model was induced by adrenaline and ice water soaking. The control group treated with the same pocedure except sc normal saline. Whole blood viscosity and plasma viscosity were evaluated by coneplate viscometer, hematocrit was determined by micro-capillary method, platelet aggregation was measured by photoelectric turbidimetry and coagulation parameters were evaluated by optical electromagnetic method. RESULTS Compared with normal control group, whole blood viscosity and plasma viscosity of rats in blood stasis model group were significantly increaed; erythrocyte aggregation index(EAI) and the platelet aggregation rate were also significantly elevated. Meanwhile, hematocrit and fibrinogen(Fib) content were also increased, while prothrombin time(PT), activated partial thromboplastin time(APTT) and thrombin time(TT) were shortened(P<0.01). Compared with model group,,hesperidin 160 and 320mg/kg could significantly decrease Fib content but delayed TT. Hesperidin 160 and 320mg/kg improved hemorheology in much the same way as aspirin, but was more effective for inhibiting EAI and platelet aggregation, reducing Fib content, prolonging TT and PT. CONCLUSION Hesperidin possesses remarkable ameriorative effects on hemorheological abnormality in rats with acute blood stasis.更多还原