【摘要】 目的采用HPLC法测定大黄中番泻苷A和番泻苷B的含量。方法色谱柱E1718897 Hypersil C18(4.6mm×250mm,25μm),流动相为四氢呋喃-水-醋酸(15∶85∶1.5),流速为0.8mL/min。结果番泻苷A的含量在0.176～1.76g/L范围内呈良好的线性关系(r=0.999 5),番泻苷B的含量在0.12～1.2g/L范围内呈良好的线性关系(r=0.999 5),平均加样回收率番泻苷A为100.44%(RSD=2.44%),番泻苷B为101.37%(RSD=2.28%)。结论该方法简便、准确、重复性好,可用于大黄药材中番泻苷A、B含量的测定。更多还原

【Abstract】 Objective To establish a method for determining sennoside A and sennoside B in Rhubarbs by HPLC.Methods sennoside A and sennoside B was analysed by using Hypersil C18column(4.6mm×250mm,25μm)with the mobile phase of tetrahydrofuran-water-acetic acid(15∶85∶1.5)at the flow rate of 0.8 mL/min,and the detective wavelength was 350nm.Results The linear range of sennoside A was from 0.176g/L to 1.76g/L(r= 0.999 5),and the linear range of sennoside B was from 0.12g/L to 1.2g/L(r=0.999 5).The average recoveries were 100.44%for sennoside A(RSD =2.44%)and 101.37%for sennoside B(RSD =2.28%).Conclusion The method is simple,accurate and suitable for the determination of sennoside A and sennoside B in Rhubarb.更多还原