【摘要】 目的探讨白藜芦醇抗动脉粥样硬化的效应及其可能机制。方法成熟雄性新西兰大白兔42只,分为三组:空白组(n=10)普通饲料喂养;模型组(n=18)高脂饲料喂养;白藜芦醇组(n=14)高脂饲料加白藜芦醇[16 mg/(kg.d)]干预。TUNEL法检测细胞凋亡,RT-PCR检测主动脉过氧化体增殖物激活型受体γ(PPARγ)、Fas、FasL及Caspase-3 mRNA表达水平,ELISA测定血清中单核细胞趋化蛋白1(MCP-1)、基质金属蛋白酶9(MMP-9)及组织型基质金属蛋白酶抑制剂1(TIMP-1)的蛋白表达水平。结果成功建立动脉粥样硬化模型。白藜芦醇组动脉粥样硬化病变明显减轻,可抑制斑块内细胞凋亡;PPARγmRNA转录增高,Fas、FasL和Caspase-3 mRNA转录显著下降;MCP-1和MMP-9蛋白表达水平显著降低,TIMP-1蛋白表达增高。结论白藜芦醇具有抗动脉粥样硬化作用,这一作用类似PPARγ激动剂,可通过上调斑块内PPARγ的基因转录,降低Fas、FasL和Caspase-3 mRNA转录,以及降低MCP-1、MMP-9的表达同时促进TIMP-1的表达来实现。更多还原

【Abstract】 Aim To explore the effect and its possible mechanism of resveratrol on atherosclerosis.Methods 42 Mature male New Zealand white rabbits were randomly divided into three groups: rabbits in blank group treated with normal diet,rabbits in model group treated with high fat diet,rabbits in resveratrol group treated with high fat diet and resveratrol at the dosage 16 mg/(kg·d).All rabbits were fed according to experiment design for 12 weeks.The aortas were harvested for histopathological examination.Apoptosis was detected by TUNEL,the mRNA transcription of PPARγ,Fas,FasL and Caspase-3 were examined by RT-PCR and MCP-1,MMP-9 and TIMP-1 were examined by ELISA. Results Atherosclerosis model was successfully established.Atherosclerotic lesions in the resveratrol group were significantly reduced.Resveratrol inhibited apoptosis of cells in the plaque.In the resveratrol group PPARγ transcription increased,Fas,FasL and Caspase-3 mRNA transcription was significantly decreased.The protein expression levels of MCP-1 and MMP-9 were significantly reduced,TIMP-1 protein expression was increased.Conclusions Resveratrol has anti-atherosclerotic role,which may be as a natural agonist through upregulating the PPARγ gene transcription within the plaque,reducing Fas,FasL and Caspase-3 transcription,and reducing the expression of MCP-1,MMP-9 while promoting TIMP-1 expression.更多还原