【摘要】 液泡型H+-ATPase(V-ATPase)在昆虫生长发育过程中具有重要作用。本文通过RT-PCR获得褐飞虱Nilaparvata lugens(Stl)V-ATPase d亚基基因NlVHA-d的cDNA序列,并通过实时荧光定量PCR对NlVHA-d基因的表达进行了分析。结果表明,NlVHA-d基因编码349个氨基酸,不同昆虫V-ATPase d亚基高度保守。NlVHA-d基因在褐飞虱2龄若虫中表达量最高,雌虫表达量显著高于雄虫表达量。LD10和LD30三唑磷处理的羽化3 d短翅雄虫NlVHA-d基因相对表达倍数分别是丙酮处理的2.15和2.46倍。亚致死剂量三唑磷处理褐飞虱短翅雄虫NlVHA-d基因的表达上调可能与褐飞虱再猖獗相关。更多还原

【Abstract】 The vacuolar H+-ATPase( V-ATPase) plays an important role in the growth and development of insects. In this study,a cDNA sequence of the V-ATPase d subunit gene was obtained from the brown planthopper,Nilaparvata lugens( Stl),by RT-PCR and named as NlVHA-d,and its expression was analyzed by qRT-PCR. The results show that the open reading frame of NlVHA-d is 1 047 bp in length,encoding 349 amino acid residues,and that the V-ATPase d genes are highly conserved among different insects. The qRT-PCR analysis revealed that the mRNA expressions levels level of NlVHA-d was the highest in second instar nymphs,and that female adults showed higher levels of expression than male adults. Furthermore,at third day after eclosion,the relative expressions levels of NlVHA-d in brachypterous male adults treated with LD10 and LD30 doses of triazophos were 2. 15 and 2. 46 times higher respectively than in controls treated with acetone. The over-expression of the NlVHA-d gene in brachypterous male adults treated with a subleathal dose of triazophos might contribute to the resurgence of the brown planthopper.更多还原