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妇淋颗粒治疗绝经期泌尿系感染的实验研究
Treatment of menopausal urinary infection with Fulin Granules
【摘要】 目的观察妇淋颗粒对去势小鼠泌尿系感染的防治作用。方法采用体外抑菌实验测定妇淋颗粒对金黄色葡萄球菌(26076)、乙型链球菌(32204)、大肠杆菌、绿脓杆菌(10105)、肺炎克雷伯杆菌(46108)、白色念珠菌的最低抑菌浓度(MIC);采用雌性去势小鼠膀胱内注入致病性大肠杆菌制备实验性泌尿道感染,分别连续灌胃妇淋颗粒(高、中、低剂量22、11、5.5 g/kg)11 d,并设立对照组,观察各组去势小鼠的阴道涂片、阴道角化细胞、小鼠血清雌二醇(E2)水平和膀胱组织学病理变化。结果妇淋颗粒对于金黄色葡萄球菌(26076)、乙型链球菌(32204)、大肠杆菌、绿脓杆菌(10105)、肺炎克雷伯杆菌(46108)、白色念珠菌均有不同程度的抑制或杀灭作用,其MIC值均远小于三金片对照组。阴道涂片显示妇淋颗粒中、高剂量组呈现出类动情前期表现;血清E2的结果表明,妇淋颗粒高、中剂量组可提高去势小鼠血清E2水平(P<0.01,P<0.05);膀胱病理组织学观察表明,妇淋颗粒高、中、低剂量组均对尿路感染小鼠的膀胱病理组织学改变有明显的治疗作用,而3种剂量组结果间无显著差异。结论妇淋颗粒对绝经期泌尿系感染小鼠具有一定的治疗作用。
【Abstract】 Objective To observe the preventive and therapeutic effects of Fulin Granules on urinary infection in mice after castration. Methods The minimum inhibitory concentration( MIC) of Fulin Granules was determined on Staphylococcus aureus( 26076),beta streptococcus( 32204),Escherichia coli,Pseudomonas aeruginosa( 10105),Klebsiella Pneumoniae( 46108) and Candida albicans by applying in vitro bacteriostasis test. The model of experimental urinary infection was established through the intravesical injection of pathogenic Escherichia coli in female castrated mice. Fulin Granules( 22、11、 5. 5 g / kg) was orally administrated in all groups for 11 days,and the changes of vaginal smears,vaginal keratinized cells,serum level of estradiol( E 2) and bladder histopathology were observed. Results Fulin Granules has inhibitory or killing effects in varying degrees on Staphylococcus aureus( 26076),beta streptococcus( 32204),Escherichia coli,Pseudomonas aeruginosa( 10105),Klebsiella Pneumoniae ( 46108) and Candida albicans,and its MIC value was much lower than that in Sanjin Pian group. The results of vaginal smears showed that there were proestrus performances in mid-dose group and high-dose group. The level of serum E 2 was reduced in high-dose group and mid-dose group( P < 0. 01,P < 0. 05),and the changes of bladder histopathology were significantly relieved in high-dose group, mid-dose group and low-dose group but there was no significant difference among these three groups. Conclusion Fulin Granules has certain therapeutic effect on menopausal urinary infection in mice.
- 【文献出处】 北京中医药大学学报 ,Journal of Beijing University of Traditional Chinese Medicine , 编辑部邮箱 ,2013年11期
- 【分类号】R277.5
- 【被引频次】13
- 【下载频次】136