Objective To provide the material foundation for inducing pluripotent stem cells through protein transduction by constructing the recombinant expression vector TAT-OCT4 which can highly express and purify fusion protein in E.coli BL21.Methods The coding human OCT4 gene sequence was obtained by RT-PCR amplification and linked to the prokaryotic expression vector TAT-V2 for the construction of recombinant vector TAT-OCT4.E.coli BL21 was transformed into TAT-OCT4.Expression of TAT-OCT4 was induced with IPTG an...
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