【摘要】 目的制备局灶性脑缺血再灌注损伤大鼠模型,并观察大脑皮质细胞色素C(CytC)与Bax蛋白表达的变化。方法采用随机数字表法将22只雄性SD大鼠分为正常组(6只)、假手术组(6只)和模型组(10只)。采用线栓法闭塞大脑中动脉2h后进行再灌注3d,制备局灶性脑缺血再灌注损伤模型。采用神经缺失评分观察大鼠的行为学表现;TTC染色检查脑组织梗死情况;HE染色观察大鼠脑组织形态结构;Western blot技术检测大鼠大脑皮质CytC与Bax蛋白的表达。结果假手术组大鼠无神经功能障碍表现,脑组织未见梗死灶,脑组织神经细胞形态规则;大脑皮质CytC与Bax蛋白的相对表达量,与正常组相比差异无统计学意义(P>0.05)。与假手术组相比,模型组大鼠出现神经功能障碍,左侧半球可见梗死灶,梗死侧脑组织形态学观察可见神经细胞大量坏死脱落,胞质呈空泡变性、疏松,胞核浓缩深染;大脑皮质CytC与Bax蛋白表达量明显增加(P<0.05)。结论大脑中动脉闭塞2h后进行再灌注3d可造成脑缺血再灌注损伤,可能与大脑皮质CytC与Bax蛋白的表达增加有关。更多还原

【Abstract】 Objective To establish a rat model of focal ischaemia-reperfusion injury,and to observe the changes in cytochrome C and Bax protein expression in cerebral cortex.Methods Twenty-two male SD rats were randomly divided into three groups:normal group(n=6),sham-operated group group(n=6) and model group(n=10).Focal cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion for 2 hours and reperfusion for 3 days.Behavioral performance,brain infarction,brain morphology and cortical expression of cytochrome C and Bax protein were measured using Neurologic Deficit Score,TTC staining,HE staining and Western blotting,respectively.Results Rats in sham-operated group showed regular neuronal cell morphology without neurological dysfunction and brain infarction.There were no significant differences in cortical expression of cytochrome C and Bax protein between sham-operated group and normal group(P>0.05).Compared with sham-operated group,cortical expression of cytochrome C and Bax protein significantly increased in model group(P<0.05).In addition,rats in model group showed neurological dysfunction,left hemisphere infarction,neuronal cell necrosis,cytoplasmic vacuolar degeneration,and deeply stained nucleus.Conclusion Middle cerebral artery occlusion(MCAO) for 2 hours and reperfusion for 3 days can induce cerebral ischemia-reperfusion injury,which may be correlated with the increase in the expression of cytochrome C and Bax protein.更多还原