节点文献
猪Lipin基因克隆及其组织分布的研究
Molecular Cloning and Tissue Distribution of Gene Lipin in Pig
【摘要】 采用逆转录PCR、电泳检测等方法,成功克隆得到了猪的Lipin 1、Lipin 2和Lipin 3基因。这3个基因与小鼠的相应基因均具有较高的同源性,达83.2%~86.7%。组织分布结果显示:Lipin 1广泛分布在猪的多种组织中,但Lipin 2和Lipin 3仅在肌肉、肺、回肠和脾中有分布。
【Abstract】 The genes Lipin 1,Lipin 2 and Lipin 3 in pig were successfully cloned by using the methods of RT- PCR and electrophoresis detection. The sequence of these three genes in pig had higher homology with that of the corresponding gene in mice,reaching 83. 2% ~ 86. 7%. The analysis of tissue distribution showed that Lipin 1 ubiquitously expressed in various pig tissues tested,but Lipin 2 and Lipin 3 only expressed in the spleen,lungs,lleum and muscle.
【关键词】 猪;
Lipin基因;
克隆;
序列分析;
组织分布;
【Key words】 Lipin gene; Clone; Sequence analysis; Tissue distribution;
【Key words】 Lipin gene; Clone; Sequence analysis; Tissue distribution;
【基金】 农业部948重点项目“猪应激相关基因功能研究及分子标记育种技术的引进”(2011-G35)
- 【文献出处】 江西农业学报 ,Acta Agriculturae Jiangxi , 编辑部邮箱 ,2013年11期
- 【分类号】S828
- 【被引频次】1
- 【下载频次】68