【摘要】 同时用0、0.21、0.30、0.43、0.60、0.81 mg/L Cu2+刺激黄河鲤,于作用2、4、6、8、10 d后采集肝胰脏取样,应用半定量RT-PCR法监测肝胰脏中铜蓝蛋白mRNA表达量的变化,来探究水体铜对黄河鲤肝胰脏铜蓝蛋白表达量的影响。结果表明:黄河鲤在不同的水体铜浓度刺激下,肝胰脏中CP mRNA的表达量对Cu2+的刺激呈现时间依赖性以及剂量依存性,随着时间变化CP mRNA表达量存在先升后降的趋势;并且CP mRNA表达量可达到一个最高峰,但达到峰值的时间不尽相同。更多还原

【Abstract】 In order to explore the effect of water copper on the content of hepatopancreas CP mRNA in Cyprinus carpio,some Cyprinus carpio were stimulated by 0,0.21,0.30,0.43,0.60,0.81 mg / L of Cu2 + at the same time,and on the 2,4,6,8,10th day after treatment,the contents of hepatopancreas CP mRNA were respectively monitored by using semi-quantitative RTPCR method.The results indicated that: under the simulation of different concentrations of copper in water body,the expression of CP mRNA in the liver and pancreas of Cyprinus carpio showed a time dependence and a dose dependence.Along with the change of the time,CP mRNA expression at first increased and then decreased;the CP mRNA expression could reach a peak,but the peak time was not the same.更多还原