【摘要】 目的观察不同输液方案对兔肺组织水通道蛋白5(AQP5)表达的影响。方法清洁级成年雄性SD家兔54只,随机分为3组(n=18):限制性输液组(A组,输液速度5 ml/h),常规输液组(B组,输液速度10 ml/h),急性高容量血液稀释组(C组,输液速度20 ml/h)。各组动物分别于输液前(T0)、输液后30 min(T1)、输液后60 min(T2)、输液后120 min(T3)记录心率(HR)、平均动脉压(MAP)、中心静脉压(CVP)等血流动力学指标。将A组在T1、T2、T3时刻再分为3组(n=6):A0.5组(T1)、A1.0组(T2)、A2.0组(T3),B、C组再分组时刻同A组。然后,分别于T1、T2、T3时刻处死动物,取相同部位右肺组织制作HE切片,光镜观察肺组织结构变化;同时取肺组织标本于透射电镜下观察肺组织超微结构变化。免疫组化染色检测AQP5的表达,图像分析测定AQP5染色的平均灰度和阳性单位。结果光镜下各组实验动物肺组织无病理学改变;透射电镜观察各组实验动物肺组织超微结构无变化。免疫组化和图像分析结果显示,肺泡Ⅰ型上皮细胞有大量AQP5阳性染色;不同输液方式对AQP5平均灰度和阳性单位的影响差异无统计学意义(P﹥0.05)。结论不同输液方式对肺组织AQP5的表达无显著影响。更多还原

【Abstract】 Objective To observe the effect of different infusion modes on the expression of aquaporin-5 in the lung of rabbits.Methods Fifty-four healthy adult male Clean SD rabbits were randomly divided into 3 groups(n=18): restricted fluid administration group(group A,transfusion speed 5 ml/h);conventional fluid administration group(group B,10 ml/h) and AHH(group C,15 ml/h).HR,MAP,CVP were recorded before infusion(T0),and 30,60,120 min after infusion(T1-3).Group A was divided into 3 subgroups(n=6) at T1,T2,T3: group A0.5,group A1.0 and group A2.0.Group B and C were divided in the same way.The rabbits were sacrificed,and the same part of pulmonary tissues was taken out at T1-3.The changes of lung tissues were observed under a light microscope and transmission electron microscope.The expression of AQP5 was determined by immunohistochemical staining test,while the value of the average gray scale and positive unit was determined by image analysis.Results There was no difference in lung tissues under the light microscope or transmission electron microscope between the groups.A large amount of AQP5 positive staining was found in Type I alveolar epithelial cells by immunohistochemistry and image analysis technology.No difference was found in the value of A and positive unit of AQP5 in different infusion modes(P>0.05).Conclusion Different infusion modes have no effect on the expression of AQP5 in the lung of rabbits.更多还原