【摘要】 为明确鸭肝脏胰岛素样生长因子1(IGF-1)及IGF-1受体(IGF-1R)基因mRNA表达的发育性变化规律,选择0、14、28、42和56日龄高邮鸭和金定鸭各10只(共100只),称测体重后屠宰,采集肝脏组织,采用实时荧光定量PCR法检测IGF-1和IGF-1R基因的发育性变化,并进行品种间比较。结果表明:0～56日龄期间,高邮鸭和金定鸭肝脏IGF-1基因表达量均呈先升后降的趋势,56日龄时高邮鸭肝脏IGF-1基因表达量极显著高于金定鸭;IGF-1R基因表达量基本呈下降趋势,高邮鸭肝脏IGF-1R基因表达量在0日龄时显著高于金定鸭。高邮鸭和金定鸭肝脏IGF-1和IGF-1R基因表达的发育性变化与体重存在不同程度的负相关性。鸭肝脏中IGF-1和IGF-1R基因表达量有特定的发育模式且存在品种差异,IGF-1R基因表达水平的变化不依赖IGF-1基因表达水平。更多还原

【Abstract】 This study was to find out the developmental patterns of insulin-like growth factor-1(IGF-1) and IGF-1 receptor(IGF-1R) gene expression in different duck breeds in liver tissue.Real time PCR was applied to investigate and compare the developmental patterns of IGF-1 and IGF-1R gene in liver tissue of duck.10 of Gaoyou duck and Jinding duck were sampled at the age of 0,14,28,42,56 day respectively.The results showed that the expression levels of IGF-1 mRNA in liver tissue in Gaoyou duck and Jinding duck increased first and declined thereafter gradually during 0 to 56 day.The expression of IGF-1 gene in Gaoyou duck had very significant difference with that in Jinding duck at the age of 56 day(P<0.01).The expression levels of IGF-1R mRNA in liver tissue declined gradually from the age of 0 to 56 day.The expression of IGF-1R gene in Gaoyou duck at the age of 0 day was significantly higher than that in Jinding duck.The developmental patterns of IGF-1 and IGF-1R gene expression in Gaoyou duck and Jinding duck had negative correlation in various degrees with body weight.The IGF-1 and IGF-1R mRNA expression in liver tissue of duck followed specific developmental patterns respectively.Level of IGF-1R mRNA expression did not depend on that of IGF-1 mRNA expression.更多还原