【摘要】 为探索Cry1Ac毒蛋白质与亚洲玉米螟[Ostrinia furnacalis(Güenée)]体内解毒酶间的关系及Cry1Ac作用下保护酶间的平衡关系,室内配制不同Cry1Ac毒蛋白质含量(200 ng/g、500 ng/g、800 ng/g、1 100 ng/g)的人工饲料饲喂亚洲玉米螟幼虫,用分光光度法测定了3龄和5龄幼虫体内乙酰胆碱酯酶、α-乙酸萘酯酶、羧酸酯酶、谷胱甘肽-S-转移酶、超氧化物歧化酶和过氧化氢酶的活性。结果表明:1 100 ng/g毒蛋白质处理的3龄幼虫乙酰胆碱酯酶活性显著高于对照和其他处理,800 ng/g处理的该酶活性显著高于200 ng/g处理,1 100 ng/g毒蛋白质处理的5龄幼虫的该酶活性显著高于200 ng/g处理。1 100 ng/g处理3、5龄幼虫的α-乙酸萘酯酶活性均显著低于对照和其他处理,800 ng/g处理的3龄幼虫该酶活性显著低于对照和200 ng/g处理,500 ng/g处理的该酶活性显著低于200 ng/g处理。1 100 ng/g、800 ng/g、500 ng/g处理的3龄幼虫羧酸酯酶活性显著低于对照,1 100 ng/g处理的5龄幼虫该酶活性显著低于对照和200 ng/g处理。1 100 ng/g、800 ng/g、500 ng/g处理的3龄幼虫谷胱甘肽转移酶活性显著低于200 ng/g处理,1 100 ng/g处理的5龄幼虫该酶活性显著低于对照。500 ng/g、800 ng/g处理3龄幼虫的超氧化物歧化酶活性显著高于对照。500 ng/g、800 ng/g、1 100 ng/g处理的3龄幼虫过氧化氢酶活性显著低于对照,800 ng/g、1 100 ng/g处理的该酶活性显著低于200 ng/g处理,1 100 ng/g处理的5龄幼虫该酶活性显著低于对照和200 ng/g处理。可见,不同含量Cry1Ac毒蛋白质处理的亚洲玉米螟体内,除5龄幼虫超氧化物歧化酶外,其他酶的活性在处理间均有显著变化。更多还原

【Abstract】 In order to discuss the relationship between Cry1Ac toxin and detoxification enzymes,and the balance of protective enzymes affected by Cry1Ac in Asian corn borer,Ostrinia furnacalis(Guenée),the activities of acetylcholinesterase,α-naphthyl acetate esterase,carboxylesterase,glutathione-S-transferase,superoxide dismutase and catalase in 3rd and 5th instar larvae of the Asian corn borer feeding on artificial diets with different contents(200 ng/g,500 ng/g,800 ng/g,and 1 100 ng/g) of Cry1Ac toxin were measured by spectrophotometry in the laboratory.The activity of acetylcholinesterase in 3rd instar larvae with the treatment of 1 100 ng/g Cry1Ac was significantly higher than those of control and other treatments.The activity of acetylcholinesterase in 3rd instar larvae in 800 ng/g Cry1Ac treatment was significantly higher than that in 200 ng/g Cry1Ac treatment,and the activity in 5th instar larvae fed with 1 100 ng/g Cry1Ac was significantly higher than that with 200 ng/g Cry1Ac.The activities of α-naphthyl acetate esterase in 3rd and 5th instar larvae with the treatment of 1 100 ng/g Cry1Ac were significantly lower than those of control and other treatments,and the activity of 3rd instar larvae with the treatment of 800 ng/g Cry1Ac was significantly lower than those of control and 200 ng/g Cry1Ac treatment,while the activity of 3rd instar larvae with 500 ng/g Cry1Ac treatment was significantly lower than that with 200 ng/g Cry1Ac treatment.The carboxylesterase activities of 3rd instar larvae in treatments of 1 100 ng/g,800 ng/g and 500 ng/g Cry1Ac were significantly lower than that of control,and the activity in 5th instar larvae with 1 100 ng/g Cry1Ac treatment was significantly lower than those of control and 200 ng/g Cry1Ac treatment.The activities of glutathione-S-transferase in 3rd instar larvae with the treatments of 1 100 ng/g,800 ng/g and 500 ng/g Cry1Ac were significantly lower than that with 200 ng/g Cry1Ac treatment,and the activity in 5th instar larvae with 1 100 ng/g Cry1Ac treatment was significantly lower than that of control.The superoxide dismutase activities of 3rd instar larvae with the treatments of 500 ng/g and 800 ng/g Cry1Ac were significantly higher than that of control.The catalase activities in 3rd instar larvae with the treatments of 500 ng/g,800 ng/g and 1 100 ng/g Cry1Ac were significantly lower than that of control,while the activities in 3rd instar larvar with 800 ng/g and 1 100 ng/g Cry1Ac treatments were significantly lower than that with 200 ng/gCry1Ac treatment,and the activity in 5th instar larvae with 1 100 ng/g Cry1Ac treatment was significantly lower than those of control and 200 ng/g Cry1Ac treatments.Thus it is conclued that the enzyme activities were significantly different among each treatment except superoxide dismutase of 5th instar larvae when the Asian corn borer feeding on artificial diets with various contents of Cry1Ac.更多还原