【摘要】 目的通过对肝脏、肌肉及皮下不同部位兔VX2肿瘤模型的建立和比较,寻求更适合于肿瘤经皮介入治疗的荷瘤动物模型。方法将54只新西兰白兔按VX2肿瘤接种部位不同随机分为肝脏组、肌肉组和皮下组,每组18只。三组动物术后12、16、20 d均进行CT灌注扫描,测量肿瘤大小,每次各组分别处死6只兔进行病理观察,并对三组模型的建模手术时间、肿瘤体积及并发症等情况进行比较。结果皮下组肿瘤生长最慢,肌肉组其次,肝脏组生长最快,组间差异有统计学意义(P<0.05)。接种第12天,肝脏组肿块长径已约1 cm,而皮下组则需20 d肿块长径才达1 cm左右,肌肉组肿块生长速度介于二者之间。三组建模所用时间分别为肝脏组(5.54±0.51)min,肌肉组(1.02±0.20)min,皮下组(0.98±0.21)min,肝脏组明显较长。除了肝脏组出现腹水、肝内转移及腹壁种植外,皮下组、肌肉组均无明显影响模型的情况。结论与肝脏和皮下建模相比,兔肌肉内VX2肿瘤模型具有操作简单,成瘤稳定,肿瘤生长速度适中的优点,更适合作为兔VX2瘤的经皮介入治疗模型。更多还原

【Abstract】 Objective To find out the tumor-bearing rabbit model which is most suitable for percutaneous interventional therapy by comparing the models receiving intrahepatic,intra-muscular or subcutaneous inoculation of VX-2 tumor. Methods A total of 54 New Zealand rabbits were randomly and equally divided into three groups. Rabbits of group A(n = 18) received intra-hepatic inoculation of VX-2 tumor. Rabbits of group B(n = 18) received intra-muscular inoculation of VX-2 tumor and rabbits of group C(n = 18) were subcutaneously inoculated with VX-2 tumor. Multi-planar CT perfusion-scanning was performed on the 12th,16thand 20thday after inoculation to determine the tumor size. Every 6 rabbits from each group were sacrificed each time at the 12th,16thand 20thday after inoculation. The specimens were collected and sent for pathologic examination. The operation time for complete modeling,the tumor size and the modeling-related complications were compared among the three groups. Results The VX-2 tumor of group A grew most fast and VX-2 tumor of group C grew slowest. The difference among the three groups was statistically significant(P < 0.05). On the 12thday after inoculation the tumor size of group A was already about one cm in diameter,while in group C and group B the tumor size became one cm in diameter on the 20thday and 16thday after inoculation respectively. The operation time needed for modeling for group A,Band C was(5.54 ± 0.51) min,(1.02 ± 0.20) min and(0.98 ± 0.21) min respectively. The operation time needed for modeling of group A was the longest. Ascites,intrahepatic metastases as well as tumor implantation of abdominal wall were found in group A,while no obvious untoward events affecting VX-2 tumor model were observed in both group B and C. Conclusion Compared to intrahepatic and subcutaneous inoculation VX-2 tumor model,the establishment of intra-muscular inoculation VX-2 tumor model of rabbit is more simple in manipulation with higher tumor-formation rate,besides the growth rate of the tumor is moderate. Therefore,intra-muscular inoculation VX-2 tumor model of rabbit is most suitable for percutaneous interventional study.更多还原