【摘要】 目的:检测肺鳞癌组织中miRNA-21和癌基因C-myc的表达,探讨两者的相关性及其临床意义。方法:采用实时荧光定量PCR(RQPCR)和免疫印迹检测25例肺鳞癌组织及其相应癌旁正常组织中miRNA-21及C-myc蛋白的表达水平,分析两者与临床病理指标的关系。结果:肺鳞癌组织中miRNA-21的表达及C-myc的表达均高于癌旁正常组织,在低分化肺鳞癌组织中的表达水平与高分化肺鳞癌组织相比,差异无统计学意义。miRNA-21及C-myc在转移癌组织中表达水平显著高于非转移癌组织。肺鳞癌组织巾miRNA-21表达量与C-myc阳性表达存在正相关。结论:miRNA-21和C-myc在肺鳞癌中表达上调,可能与肺癌的发生与演进相关。更多还原

【Abstract】 Objective:To investigate the expression and clinical significance of miRNA-21 and C-myc protein in lung squamous carcinoma tissues.Methods:Quantitative real-time PCR(qRT-PCR) and Western blotting were used to detect miRNA-21 and C-myc protein levels in 25 cases of lung squamous carcinoma and corresponding adjacent normal tissues.The relationship between expressional levels and pathological characters was analyzed.Results:The expression levels of miRNA-21 and C-myc in lung squamous carcinoma were significantly higher than those in the normal adjacent tissues.The expression level of miRNA-21 and C-myc between poorly differentiated tissues and well differentiated tissues in lung squamous cell carcinoma was not statistically significant.The expression level of miRNA-21 and C-myc in metastatic carcinoma was higher than that in non metastatic carcinoma of lung squamous cell carcinoma.The level of miRNA-21 expression was positively correlated with Cmyc protein expression.Conclusion:Up-regulated expressions of miRNA-21 and C-myc protein in lung squamous cell carcinoma tissues might contribute to lung carcinogenesis.更多还原