【摘要】 研究了在液体木屑培养基中添加0.005、0.01、0.015 mg/mL浓度的氨基寡糖对3株侧耳发酵液中漆酶活性的影响,结果表明:氨基寡糖对侧耳漆酶的产生有明显地促进作用,3株侧耳菌株在氨基寡糖液体木屑培养基中均有漆酶活性,酶活随发酵时间和氨基寡糖浓度而变化,冀微961糙皮侧耳在0.01 mg/mL氨基寡糖浓度,第10 d时漆酶酶活达到最高238 U/mL,比对照增加49.7%。漆酶参与木质素的降解,对木腐菌的生长有着直接的影响,氨基寡糖通过促进漆酶活性,影响侧耳的生长发育。更多还原

【Abstract】 The effects of adding 0.005,0.01,0.015 mg/mL oligosaccharin on activity of laccase in the liquid medium containing sawdust of three stains Pleurotus sp.were studied.The results showed that three concentration of oligosaccharin all had marked effects to the activity of laccase.The most obvious effect was to ’Jiwei 961’ P.ostreatus,in which the activity of laccase in the fermented liquid reached to 238U/mL when added 0.01mg/mL oligosaccharin and cultivated for 10 days.That is 49.7% more than the contrast which no oligosaccharin was added.The laccase can accelerate the degradation of lignin,which have great effect on the growth of wood-rot fungi.Oligosaccharin can promote the growth of P.sp.by enhancing the activity of laccase.更多还原