【摘要】 14-3-3蛋白是一种可以改变其结合蛋白构象的酸性蛋白质。柞蚕14-3-3 cDNA序列全长1 220 bp,包括一个126 bp的5’非编码区和一个350 bp的3’非编码区。该基因的开放读码框长度为744 bp,编码247个氨基酸。序列比对结果表明,柞蚕14-3-3蛋白与家蚕的14-3-3蛋白具有高度同源性。此外对柞蚕14-3-3基因进行了原核表达和重组蛋白纯化。SDS-PAGE和免疫印迹结果表明,分子量大小约32 kD的重组蛋白在大肠杆菌中得到了成功表达。更多还原

【Abstract】 The 14-3-3 protein is a acidic regulatory protein modulating the conformation of its binding partner.A 14-33 gene,named as Ap-14-3-3,was identified from the Chinese Oak Silkworm Antheraea pernyi.The full-length cDNA of Ap-14-3-3 is 1 220 bp,including a 5’-untranslated region(UTR) of 126 bp,3’-UTR of 350 bp and an open reading frame(ORF) of 744 bp encoding a polypeptide of 247 amino acids.The deduced A.pernyi 14-3-3 protein sequence is highly homologous to its homologue of Bombyx mori.Prokaryotic expression and purification of the Ap-14-3-3 protein were performed,SDS-PAGE and western blot analysis demonstrated that a 32 kD recombinant protein was successfully expressed in E.coli cells.更多还原