【摘要】 目的研究蛋白激酶B/雷帕霉素靶蛋白(AKT/mTOR)在胃癌组织中的表达水平及与临床病理因素的关系。方法应用免疫组化染色测定磷酸化蛋白激酶B(p-AKT)和磷酸化雷帕霉素靶蛋白(p-mTOR)在胃癌(32例)、癌旁组织(20例)中的表达情况。结果 p-AKT在胃癌、癌旁组织中的阳性表达分别为71.9%、25.0%。p-mTOR在胃癌、癌旁组织中的阳性表达分别为62.5%、20.0%。p-AKT、p-mTOR的表达与胃癌组织分化程度、TNM分期存在相关性(P<0.05),p-AKT、p-mTOR的表达与胃癌患者的年龄、性别、肿瘤直径大小无相关性(P>0.05)。结论胃癌组织中p-AKT与p-mTOR蛋白的表达与病理分化程度呈负相关。AKT/mTOR信号通路在大部分胃癌组织中过度表达可能是胃癌发生发展的重要机制之一。更多还原

【Abstract】 Objective To explore the expression of AKT/mTOR (Protein kinase B/mammalian target of rapamycin) in gastric cancer and the relationship between the expression with the clinical and pathological factors in gastric cancer. Methods The expression of p-AKT and p-mTOR were measured in 32 samples of gastric cancer, and 20 samples of paracancer tissue by immunohistochemistry. Results The expression of p-AKT was found in 71.9% of gastric cancer and 25% of paracancer tissues. The expression of p-mTOR in gastric cancer and paracancer tissues was 62.5%,20%. The expression of p-AKT and p-mTOR in gastric cancer were correlated to the differentiation of histological tissues and T 、N、M stages (P <0.05) . The expression of p-AKT and pmTOR in gastric cancer were not related to patient’s age,gender,tumor diameter (P>0.05) . Conclusions The expression of p-AKT and p-mTOR in gastric cancer were negatively associated with the pathological differentiation level. AKT/mTOR were over expressed in the majority of gastric cancer tissue,it may play an important role in development of gastric cancer.更多还原