【摘要】 目的研究L-茶氨酸对叠氮钠(NaN3)诱导的人神经母细胞瘤(SH-SY5Y)细胞线粒体损伤的影响其机制。方法将不同浓度的L-茶氨酸及NaN3与SH-SY5Y细胞系共同孵育,用MTT法测定细胞存活力,以JC-1测定法和激光共聚焦显微镜来观察线粒体膜电位,Western blot法检测细胞内caspase-3、caspase-9和细胞色素c蛋白表达水平。结果 1)随着NaN3浓度的增高,细胞活性逐步降低,提前2 h给予茶氨酸(0.25、0.50和1.00 mmol/L)能明显降低NaN3(0.25 mmol/L)孵育24 h对细胞的毒性并逆转线粒体跨膜电位的下降(P<0.05);2)茶氨酸在0.25~1.00 mmol/L浓度范围内能显著逆转NaN3诱导的SH-SY5Y细胞内caspase-3、caspase-9和细胞色素c蛋白表达水平的升高(P<0.05)。结论茶氨酸可以通过稳定SH-SY5Y细胞线粒体膜电位,减轻细胞线粒体的损伤。更多还原

【Abstract】 Objective To investigate the effects and its mechanism of L-theanine on mitochondrial injury induced by sodium azide in SH-SY5Y cells.Methods The SH-SY5Y cells were exposed to different concentrations of L-theanine and the sodium azide,the cell viability was measured by the MTT method,the mitochondrial membrane potential was detected by the JC-1 method and the Scanning Confocal Microscopy.The protein level of intracellular caspase-3,caspase-9 and cytochrome c was detected by the Western Blotting Method.Results 1) With the increase of the concentration of sodium azide,the cell activity decreases gradually.After pretreatment by L-theanine(0.25,0.50,1.00 mmol/L) for 2 hours,it significantly reduced cell toxicity and reversed decline in the mitochondrial membrane potential induced by the sodium azide(0.25 mmol/L) for 24 hours(P<0.05).2) L-theanine(0.25~1.0 mmol/L) significantly reversed the increasing of the protein expression level of the caspase-3,caspase-9 and cytochrome c induced by the sodium azide in SH-SY5Y cell(P<0.05).Conclusions L-Theanine can stabilize mitochondrial membrane potential of the SH-SY5Y cell and thus reduce the mitochondrial damage.更多还原