【摘要】 目的探讨血红素加氧酶-1(HO-1)对自发性高血压大鼠(SHR)肝脏基质金属蛋白酶-2(MMP-2)与-9(MMP-9)表达的影响。方法大鼠随机分为4组:Wistar诱导组(WH)与SHR诱导组(SH)按15 mg/kg每天连续4天腹腔内注射浓度为8 g/L的氯化血红素(hemin)诱导HO-1表达;Wistar对照组(W)与SHR对照组(S)腹腔内注射等体积0.1 mol/L NaOH(pH 8.3)。检测大鼠尾动脉收缩压。免疫组化法分析肝脏HO-1及MMPs表达。明胶酶谱法检测血浆MMP-2及MMP-9的相对活性。结果 WH组与SH组肝脏HO-1、MMP-2与MMP-9表达量均显著高于W组与S组。HO-1诱导可显著降低SHR大鼠血压。S组血浆MMP-2及MMP-9相对活性显著高于W组。WH组与SH组血浆MMP-2及MMP-9相对活性显著高于W组与S组。结论 HO-1可上调SHR肝脏MMP-2与MMP-9表达。更多还原

【Abstract】 Objective To explore the effects of hemeoxygenase-1(HO-1) induction on matrix metalloproteinase 2(MMP-2) and MMP-9 expression in liver of spontaneously hypertensive rats(SHR).Methods Rats were randomly divided into 4 groups.Wistar induction group(WH) and SHR induction group(SH) : 8 g / L hemin was administered to the animals intraperitoneally at a concentration of 15 mg / kg.d for 4 consecutive days to induce expression of HO-1;Wistar control group(W) and SHR control group(S) : equal volume of 0.1 mol / L NaOH(pH 8.3) was administered intraperitoneally as controls.Systolic blood pressure(BP) of rats was determined.The expressions of HO-1,MMP-2 and MMP-9 in liver were determined by immunohistochemistry.The relative activity of MMP-2 and MMP-9 in plasma were detected by gelatin zymography.Results The expression of liver HO-1,MMP-2 and MMP-9 was significantly higher in WH and SH group as compared with W and S group,respec-tively(P < 0.01).Induction of HO-1 significantly attenuated BP of SHR.The relative activity of MMP-2 and MMP-9 in rat plasma was significantly higher in S group as compared with W group(P < 0.01).The relative activity of MMP-2 and MMP-9 in rat plasma was significantly higher in WH and SH group compared with W and S group,respectively(P < 0.01).Conclusions HO-1 up-regulates expressions of MMP-2 and MMP-9 in liver of SHR and the mechanisms need to be further elucidated.更多还原