【摘要】 目的探讨骨形态发生蛋白9(BMP9)过表达对人骨肉瘤细胞系143B的生物学行为的影响。方法用RT-PCR和Western blot检测骨肉瘤细胞系中BMP9的内源性表达,用表达BMP9的腺病毒重组体(adBMP9)感染内源性表达BMP9相对较低的骨肉瘤细胞系,Transwell法检测细胞侵袭,划痕愈合实验检测细胞迁移,MTT法和结晶紫染色法检测细胞的增殖,Hoechst 33258染色法检测凋亡,平板集落形成实验检测集落形成能力。结果 adBMP9可以明显减弱骨肉瘤细胞系143B的增殖、迁移、侵袭及集落形成能力(P<0.05),并促进细胞凋亡。结论 adBMP9能抑制人骨肉瘤细胞系143B的增殖和迁移,促进细胞凋亡。更多还原

【Abstract】 Objective To investiage the effect of bone morphogenetic protein 9(BMP9) on the biological behavior of human osteosarcoma cell line 143B.Methods Using RT-PCR and Western blot annlysis to characterize the endogenous expression of BMP9 in the osteosarcoma cell line.Using a recombinant adenovirus expressing BMP9(adBMP9) to infect the osteosarcoma cell line with relatively low endogenous BMP9,then Transwell assay was used to determine cell invasion;Wound-healing assay was used to determine cell migration;MTT assay and crystal violet staining were used to determine the influence of adBMP9 on osteosarcoma cell proliferarion;Hoechst 33258 assay was used to determine cell apoptosis;Clonoy formation assay was used to determine cell clonoy.Results Osteosarcoma cell proliferation,migration,invasion and colony formation were significantly decreased while the cell apoptosis was induced by adBMP9.Conclusions adBMP9 is able to inhibit cell proliferation as well as migration and also induce cell apoptosis in human osteosarcoma cell line 143B.更多还原