【摘要】 目的比较荧光定量PCR法(RT-PCR)和原位杂交法检测头颈部鳞状细胞癌中HPV(HPV)16/18亚型感染的差异。方法采用RT-PCR法和原位杂交法对78例头颈部鳞状细胞癌患者的肿瘤组织进行HPV感染状态的检测,评价两种方法的一致性。结果 RT-PCR法检测到62.8%的头颈部鳞癌组织中含有HPV16/18 DNA。原位杂交法检测到47.4%的肿瘤组织中有HPV16 DNA。用RT-PCR方法,唇、口腔、口咽及下咽的HPV16/18 DNA阳性率分别为33.3%、66.67%、70%和57.14%;用原位杂交方法,唇、口腔、口咽、下咽的HPV16 DNA阳性率分别为33.3%、43.8%、60.0%和57.1%。总体上,两种方法检测HPV16/18DNA具有较高一致性(Kappa=0.595,P<0.001)。结论 RT-PCR和原位杂交法检测HPV16/18 DNA结果的一致性较高。更多还原

【Abstract】 Objective To compare the effican of two different methods,real time PCR(RT-PCR) and in situ hybridization,in detecting human papillomavirus 16/18(HPV16/18) infection in the tissue of head and neck squamous cell cancer.Methods The level of HPV16/18 DNA was measured by RT-PCR and in situ hybridization in the tissues from head and neck squamous cell cancer(n=78).Results The positive rates of HPV infection were 62.8% by RT-PCR and 47.4% by in situ hybridization(ISH).By RT-PCR,the incidence rates of HPV16/18 DNA positive in lip,oral cavity,oropharynx and hypopharynx were 33.3%,66.7%,70% and 57.1%,respectively.By ISH,the positive rates of HPV16/18 DNA in corresponding location were 33.3%,43.8%,60.0% and 57.1%,respectively.In general,these two methods had a high consistency in detecting HPV infection(kappa=0.595,P<0.001).Conclusions RT-PCR and in situ hybridization have a high consistency in detecting HPV16/18 DNA infection in the cancer tissues.Both of them are qualified for evaluating HPV16/18 infection status in head and neck squamous cell cancer tissues.更多还原