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下调Notch1信号抑制人神经母细胞瘤SH-SY5Y的增殖能力

Down-regulation of Notch-1 signaling contributes to cell growth inhibition in human neuroblastoma SH-SY5Y cells

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【作者】 郑志竑林玲张燕郑莺凤

【Author】 ZHENG Zhi-hong,LIN Ling,ZHANG Yan,ZHENG Ying-feng(Dept.of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Fujian Medical University,Fuzhou 350004,China)

【机构】 福建医科大学基础医学院生物化学与分子生物学系杭州艾迪康医学检验中心加拿大Manitoba大学

【摘要】 目的探讨Notch1基因与人神经母细胞瘤SH-SY5Y细胞增殖能力的关系。方法应用-分泌酶抑制剂DAPT或表达Notch1-shRNA慢病毒载体阻断SH-SY5Y细胞Notch1信号的活化,RT-PCR和Western blot法行Notch1及Hes1表达水平的检测,MTT法和裸鼠体内种植检测瘤细胞体内外增殖能力的变化。结果 DAPT处理和表达Notch1-shR-NA慢病毒载体转染均能减少细胞内NICD和Hes1表达,其细胞体外增殖能力受到明显抑制(P<0.01),Notch1 RNAi细胞体内种植瘤重为0.20±0.13 g,明显轻于对照组的0.89±0.58 g(P<0.01)。结论 Notch1信号的活化与人神经母细胞瘤SH-SY5Y细胞的增殖能力密切相关,有望成为神经母细胞瘤基因治疗的潜在靶点。

【Abstract】 Objective To investigate whether Notch1 gene to changes the proliferation of human neuroblastoma SH-SY5Y cell line.Methods Notch1 gene activation of SH-SY5Y cells was blocked using γ-secretase inhibiter DAPT or knockdown with recombination lentivirus which express Notch1-shRNA.RT-PCR and Western Blot were applied to monitor the validity of down-regulation of notch1 and Hes1 expression.MTT assay was performed for examining cell proliferation in vitro.The influence of Notch1 RNAi on the implanted tumor growth was observed.ResultsDAPT and the lentiviral vectors expressing Notch1-shRNA were efficient in down-regulating notch1(NICD) and Hes1 expression.The down-regulation of Notch1 gene inhibited cell proliferation significantly(P<0.01).The implanted tumor weight in Notch1-knockdown group was markedly lower than that in control group(0.20±0.13 g vs 0.89±0.58 g,P<0.01).Conclusions Notch1 gene activation,which can lead to the change of proliferation of SH-SY5Y cell line,is a potential target molecule for neuroblastoma of gene therapy.

【基金】 福建省自然科学基金(C0810017);福建医科大学研究基金(JS06008)
  • 【文献出处】 基础医学与临床 ,Basic & Clinical Medicine , 编辑部邮箱 ,2013年05期
  • 【分类号】R739.4
  • 【被引频次】6
  • 【下载频次】139
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