【摘要】 为探讨非典型气味受体基因Orco的功能,采用RT-PCR和RACE方法克隆获得桔小实蝇Bacto-cera dorsalis(Hendel)Orco受体的全长cDNA序列,命名为Bdor/Orco(GenBank登录号为EU621792)。测序结果表明Bdor/Orco开放阅读框全长1 422bp,编码473个氨基酸残基。对其组成成分、疏水/亲水区域、信号肽、蛋白质二级结构,以及分子进化关系等进行了预测与推断,分析结果表明Bdor/Orco与其他昆虫的Orco具有较高的氨基酸序列一致性,特别是C末端。对该基因在桔小实蝇成虫不同组织和发育时期表达量的荧光定量PCR分析,结果表明Bdor/Orco主要是在桔小实蝇成虫触角中表达,头部(去除触角)、雌虫前足和翅中也有较高的表达;桔小实蝇在各个发育时期的表达水平不同,在刚羽化雌成虫中的表达量最高。更多还原

【Abstract】 In order to explore the functions of Orco,the gene encoding Orco from Bactrocera dorsalis(Hendel) was cloned and named as Bdor/Orco(GenBank accession No.EU621792) by RT-PCR and RACE.The sequencing results showed that the open reading frame of Bdor/Orco was 1 422 bp in length,encoding 473 amino acid residues.The Bdor/Orco was analyzed and predicted by the tools of bioinformatics in the following aspects: the composition,hydrophobicity or hydrophilicity,the signal peptide,protein secondary structure,the composition of homologous protein and the phylogenetic relationship.The results showed Bdor/Orco had the characteristics of Orco,with special conserved C domains.Tissue expression pattern revealed that Bdor/Orco transcript was observed clearly in the antenna of B.dorsalis,and highly expressed in the head(with antennae removed),female foreleg and wing.The temporal expression pattern further indicated that Bdor/Orco was also expressed in different developmental stages at different expression levels with the highest level in newly emerged female adult.更多还原